Русский
Analysis of the microbial profile of sputum and the IL-4, IL-6, IL-10, IFN-γ cytokine levels in patients with community-acquired pneumonia during the COVID-19 pandemic
DOI: https://dx.doi.org/10.18565/epidem.2024.14.1.43-8
Polishchuk I.S., Aleshukina A.V., Dontsov D.V., Markova K.G., Berezinskaya I.S., Tverdokhlebova T.I.
1) Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; 2) Rostov State Medical University, Rostov-on-Don, Russia
Materials and methods. Patients with CAP with positive results of sputum polymerase chain reaction (PCR) for the RNA of the new coronavirus (SARS-CoV-2) were included in the group 1 (n = 46), patients with negative results - in the group 2 (n = 57). Bacteriological and enzyme immunoassays of sputum were performed.
Results. In both groups there were pathogens belonging to 3 genera: Streptococcus spp. in group 1 – 35.7 ± 2.0, in group 2 – 34.0 ± 2.2 (p > 0.05); Staphylococcus spp. – 24.5 ± 2.2 and 11.8 ± 4.3% (p < 0.05); yeast-like fungi of the Candida genus – 47.0 ± 2.3 and 11.2 ± 1.3% (p < 0.05), respectively. Analysis of cytokines in sputum showed that in samples from patients of the group 1, the IFN-γ γ level was 89.58 ± 26.5 pg/mL, and in the group 2 – 121.07 ± 24.55 pg/mL (p > 0, 05); IL-10 – 22.11 ± 6.19 pg/mL and 13.64 ± 3.50 pg/mL, respectively (p > 0.05).
Conclusion. The structure of the sputum microbiota in patients with CAP in both groups was dominated by Streptococcus and Staphylococcus, which were significantly more resistant to antibiotics. The significant role of yeast-like fungi of the Candida genus in the development of CAP has been shown. In CAP, overproduction of the anti-inflammatory IFN-γ was detected. A significant difference in cytokine levels in groups 1 and 2 was noted for IL-10 – 22.1 ± and 13.6 ± 3.5 pg/ml, respectively (p < 0.05).
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About the Autors
Inna S. Polishchuk, Junior Researcher, Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; in.polischuk2014@yandex.ru; http://orcid.org/0000-0002-7188-6465
Anna V. Aleshukina, MD, Chief Researcher, Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; aaleshukina@mail.ru; http://orcid.org/0000-0002-9797-2441
Denis V. Dontsov, Associate Professor, Professor, Department of Infectious Diseases, Rostov State Medical University, Rostov-on-Don, Russia; d_dontcov@mail.ru; http://orcid.org/0000-0001-9253-3864
Kristina G. Markova, Researcher, Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; syomchenko@rambler.ru; http://orcid.org/0000-0001-5154-1171
Iraida S. Berezinskaya, Junior Researcher, Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; aleshukina@yandex.ru; http://orcid.org/0000-0001-7503-6465; SPIN-978539
Tatiana I. Tverdokhlebova, MD, Director, Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being; Associate Professor, Rostov State Medical University, Rostov-on-Don, Russia; rostovniimp@mail.ru; http://orcid.org/0000-0002-3912-0291
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