Русский
Molecular epidemiological analysis of HIV-1 genetic variants circulating in the countries of Eastern European and Central Asia in 2010-2019
DOI: https://dx.doi.org/10.18565/epidem.2022.12.3.31-40
Lapovok I.A., Kirichenko A.A., Shlykova A.V., Saleeva D.V., Lopatukhin A.E., Kireev D.E., Ladnaia N.N., Musabaev E.I., Kazakova E.I., Ibadullaeva N.S., Ibragimova S.S., Safarova A.M., Kadirova A., Nurlyaminova Z., Ismatova L., Zukhurova M., Kholnazarov R., Bekbolotov A.A., Narmatova E.B., Iskanova B.M., Grigoryan T.R., Petrosyan A.R., Sargatyan T.A., Gasich E.L., Bunas A.S., Glinskaya I.N., Pokrovsky V.V.
1) Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Right Protection and Human Well-Being, Moscow, Russia; 2) Research Institute of Virology, Tashkent, Uzbekistan; 3) Republic Center for AIDS Control, Baku, Azerbaijan; 4) Research Institute of Pulmonary Diseases, Baku, Azerbaijan; 5) Republican Center for AIDS Prevention and Control, Dushanbe, Tajikistan; 6) Republican AIDS Center, Ministry of Health of the Kyrgyz Republic, Bishkek, Kyrgyzia; 7) Osh Regional AIDS Center, Ministry of Health of the Kyrgyz Republic, Osh, Kyrgyzia; 8) National Center for AIDS Prevention, Yerevan, Armenia; 8) Republican Center for AIDS Prevention, Ministry of Health of the Republic of Armenia, Yerevan, Armenia; 9) Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, Belarus; 10) Republican Center for Hygiene, Epidemiology, and Public Health, Minsk, Belarus
Materials and methods. Nucleotide sequences of the HIV-1 pol gene fragment (2253–3344 bp) in the plasma samples were obtained from HIV-infected patients in Russia (RU), Armenia (AM), Azerbaijan (AZ), Belarus (BY), Kyrgyzstan (KG), Tajikistan (TJ), and Uzbekistan (UZ). The collection was supplemented with nucleotide sequences from the international database.
Results. A total of 1825 nucleotide sequences from the countries studied were analyzed. Infection through unprotected heterosexual contact was the dominant route of transmission (about 50%). There was a decrease in the proportion of injecting drug users in 2010–2019. The dominant HIV-1 genetic variants were subtype A6 (64.82%), CRF63_02A1 (22.58%), and subtype B (7.40%). Moreover, the share of CRF63_02A1 in Central Asian countries was significantly higher than that in other EECA countries. There were 48 unique recombinant forms, 38 of which were AG recombinants. 140 molecular clusters were formed containing 369 (20.22%) sequences.
Conclusion. There is a need for further investigations of HIV-1 genetic diversity in the EECA region and for supervision over the generation and spread of new HIV-1 recombinant forms.
Literature
1. UNAIDS DATA 2019. https://www.unaids.org/sites/default/files/ media_asset/2019-UNAIDS-data_en.pdf 2. Los Alamos HIV database. https://www.hiv.lanl.gov/content/index 3. Bbosa N., Kaleebu P., Ssemwanga D. HIV subtype diversity worldwide. Curr Opin HIV AIDS 2019; 14(3): 153–60. DOI: 10.1097/COH.0000000000000534 4. Foley B.T., Leitner T., Paraskevis D., Peeters M. Primate immunodeficiency virus classification and nomenclature: Review. Infect. Genet. Evol. 2016; 46: 150–8. DOI: 10.1016/j.meegid.2016.10.018. 5. Díez-Fuertes F., Cabello M., Thomson M.M. Bayesian phylogeographic analyses clarify the origin of the HIV-1 subtype A variant circulating in former Soviet Union’s countries. Infect. Genet. Evol. 2015; 33: 6. Thomson M.M., Najera R. Increasing HIV-1 genetic diversity in Europe. J. Infect. Dis. 2007; 196(8): 1120–4. DOI: 10.1086/521683 7. Kostaki E.-G., Karamitros T., Bobkova M., Oikonomopoulou M., Magiorkinis G., Garcia F. et al. Spatiotemporal Characteristics of the HIV-1 CRF02_AG/CRF63_02A1 Epidemic in Russia and Central Asia. AIDS Res. Hum. Retroviruses 2018; 34(5): 415–20. DOI: 10.1089/AID.2017.0233 8. Saad M.D., Shcherbinskaya A.M., Nadai Y., Kruglov Y.V., Antonenko S.V., Lyullchuk M.G. et al. Molecular epidemiology of HIV Type 1 in Ukraine: birthplace of an epidemic. AIDS Res. Hum. Retroviruses 2006; 22(8): 709–14. DOI: 10.1089/aid.2006.22.709 9. Baryshev P., Bogachev V., Gashnikova N. HIV-1 genetic diversity in Russia: CRF63_02A1, a new HIV type 1 genetic variant spreading in Siberia. AIDS Res. Hum. Retroviruses 2014; 30(6): 592–7. DOI: 10.1089/AID.2013.019 10. Лаповок И.А., Лопатухин А.Э., Киреев Д.Е., Казеннова Е.В., Лебедев А.В., Бобкова М.Р. и др. Молекулярно-эпидемиологический анализ вариантов ВИЧ-1, циркулировавших в России в 1987–2015 гг. Тер. архив 2017; 89(11): 44–9. DOI: 10.17116/terarkh2017891144-49 Lapovok I.A., Lopatukhin A.E., Kireev D.E., Kazennova E.V., Lebedev A.V., Bobkova M.R. et al. [Molecular epidemiological analysis of HIV-1 variants circulating in Russia in 1987–2015]. Terapevticheskiy arkhiv 2017; 89(11): 44–9. (In Russ.). DOI: 10.17116/terarkh2017891144-49 11. Kazennova E., Laga V., Gromov K., Lebedeva N., Zhukova E., Pronin A. et al. Genetic Variants of HIV Type 1 in Men Who Have Sex with Men in Russia. AIDS Res. Hum. Retroviruses 2017; 33(10): 1061–4. DOI: 10.1089/AID.2017.0078 12. Bobkova M. Current status of HIV-1 diversity and drug resistance monitoring in the former USSR. AIDS Rev. 2013; 15(4): 204–12. PMID: 24192601 13. Kazennova E., Laga V., Lapovok I., Glushchenko N., Neshumaev D., Vasilyev A., Bobkova M. HIV-1 genetic variants in the Russian Far East. AIDS Res. Hum. Retroviruses 2014; 30(8): 742–52. DOI: 10.1089/AID.2013.0194. 14. Lebedev A., Pasechnik O., Ozhmegova E., Antonova A., Blokh A., Grezina L. et al. Prevalence and spatiotemporal dynamics of HIV-1 Circulating Recombinant Form 03_AB (CRF03_AB) in the Former Soviet Union countries. PLoS ONE 2020; 15(10): e0241269. DOI: 10.1371/journal.pone.0241269 15. Murzakova A., Kireev D., Baryshev P., Lopatukhin A., Serova E., Shemshura A. et al. Molecular Epidemiology of HIV-1 Subtype G in the Russian Federation. Viruses 2019; 11(4): 348. DOI: 10.3390/v11040348. 16. Саухат С.Р., Покровский В.В., Воронцов Д.В., Орлов Н.В., Рабинович В.Д., Тормозова Н.М. и др. Влияние случаев ВИЧ-инфекции, связанных с нозокомиальными очагами, на дальнейшее развитие эпидемического процесса. Эпидемиология и инфекционные болезни 2004; (4): 16–20. Saukhat S.R., Pokrovsky V.V., Voronstov D.V., Orlov N.V., Rabinovich V.D., Tormozova N.M. et al. [Impact of HIV infection cases associated with nosocomial outbreak further development of an epidemic process]. Epidemiol. Infect. Dis. 2004; (4): 16–20. (In Russ.). 17. Lebedev A., Lebedeva N., Moskaleychik F., Pronin A., Kazennova E., Bobkova M. Human Immunodeficiency Virus-1 Diversity in the Moscow Region, Russia: Phylodynamics of the Most Common Subtypes. Front. Microbiol. 2019; 10: 320. DOI: 10.3389/fmicb.2019.00320 18. Laga V., Vasilyev A., Lapovok I., Grigoryan S., Papoyan A., Glushchenko N. et al. HIV Type 1 Subtype A1 Dominates in Armenia. Current HIV Research. 2015; 13(3): 219–25. DOI: 10.2174/1570162x 13666150407142834 19. Осадчая О.А., Ерошкин П.В., Кириченко А.А., Лаповок И.А., Салеева Д.В., Лопатухин А.Э. и др. Изучение передачи лекарственной устойчивости ВИЧ-1 в Республике Армения с помощью биоинформатических методов. Эпидемиол. инфекц. болезни. Актуал. вопр. 2021; 11(3): 53–60. DOI: 10.18565/epidem.2021.11.3.53–60 Osadchaya O.A., Eroshkin P.V., Kirichenko A.A., Lapovok I.A., Saleeva D.V., Lopatukhin A.E. et al. Studying the transmission of HIV-1 drug resistance in the Republic of Armenia using bioinformatics methods. Epidemiоlоgy and infectious diseases. Current items 2021; 11(4): 53–60. (In Russ.). DOI: 10.18565/epidem.2021.11.3.53–60 20. Saad M.D., Aliev Q., Botros B.A.M., Carr J.K., Gomatos P.J., Nadai Y. et al. Genetic Forms of HIV Type 1 in the Former Soviet Union Dominate the Epidemic in Azerbaijan. AIDS Res. Hum. Retroviruses 2006; 22(8): 796–800. DOI: 10.1089/aid.2006.22.79 21. Botros B.A., Aliyev Q.M., Saad M.D., Michael A.A., Sanchez J.L., Carr J.K., Earhart K.C. HIV infection and associated risk factors among long-distance truck drivers travelling through Azerbaijan. Int. J. STD. AIDS 2009; 20(7): 477–82. DOI: 10.1258/ijsa.2008.008396 22. Lazouskaya N.V., Eremin V.F., Adema K.W., Gasich E.L., Baan E., Lukashov V.V. The HIV Type 1 Epidemic in Belarus: Predominance of Eastern European Subtype A Strains and Circulation of Subtype B Viruses AIDS Res. Hum. Retroviruses 2005; 21(9): 830–3. DOI: 10.1089/aid.2005.21.83 23. Sivay M.V., Totmenin A.V., Zyryanova D.P., Osipova I.P., Nalimova T.M., Gashnikova M.P. et al. Characterization of HIV-1 Epidemic in Kyrgyzstan. Front. Microbiol. 2021; 12: 753675. DOI: 10.3389/fmicb.2021.753675 24. Kirichenko A., Kireev D., Lopatukhin A., Murzakova A., Lapovok I., Saleeva D. et al. Prevalence of HIV-1 drug resistance in Eastern European and Central Asian countries. PLoS ONE 2022; 17(1): e0257731. DOI: 10.1371/journal.pone.0257731 25. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol. 2013; 30(12): 2725–9. DOI: 10.1093/molbev/mst197 26. jpHMM (jumping profile Hidden Markov Model). http://jphmm.gobics.de/ 27. Герасимов А.Н. Медицинская статистика: учебное пособие для студентов медицинских вузов. М.: МИА, 2007. 475 с. Gerasimov A.N. Medical statistics: a textbook for medical students. Moscow, 2007. 475 p. (In Russ.). 28. Detecting and Responding to HIV Transmission Clusters. A Guide for Health Departments. June 2018. Draft Version 2.0. https://www.cdc.gov/hiv/pdf/funding/announcements/ps18-1802/cdc-hiv-ps18-1802-attachmente-detecting-investigating-and-responding-to-hiv-transmission-clusters.pdf 29. Aibekova L., Foley B., Hortelano G., Raees M., Abdraimov S., Toichuev R., et al. Molecular epidemiology of HIV-1 subtype A in former Soviet Union countries. PloS ONE 2018; 13(2): e0191891. DOI: 10.1371/journal.pone.0191891
About the Autors
Ilya A. Lapovok, Cand. Biol. Sci., Senior Researcher, Central Research Institute of Epidemiology, Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; i_lapovok@mail.ru; http://orcid.org/0000-0002-6328-1415
Alina A. Kirichenkо, Researcher, Central Research Institute of Epidemiology, Federal Service for the Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; kotova-kirichenko@mail.ru; http://orcid.org/0000-0002-7116-0138
Anastasia V. Shlykova, Researcher, Central Research Institute of Epidemiology, Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; kotova-kirichenko@mail.ru; http://orcid.org/0000-0002-1390-8021
Daria V. Saleeva, Researcher, Central Research Institute of Epidemiology, Federal Service for the Supervision of Consumer Rights Protection and Human Well-Being; Researcher, State Research Center – Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Moscow, Russia; http://orcid.org/0000-0002-5870-5594
Alexey E. Lopatukhin, Researcher,, Central Research Institute of Epidemiology, Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; a.lopatukhin@gmail.com; http://orcid.org/0000-0002-2826-699X
Dmitry E. Kireev, Cand. Biol. Sci., Head, Laboratory, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Right Protection and Human Well-Being,, Moscow, Russia; dmitkireev@yandex.ru; http://orcid.org/0000-0002-7896-2379
Natalya N. Ladnaia, Cand. Biol. Sci., Senior Researcher, Central Research Institute of Epidemiology, Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; n.ladnaia@hiv-russia.ru; http://orcid.org/0000-0003-2994-151X
Professor Erkin I. Musabaev, MD, Director, Research Institute of Virology, Tashkent, Uzbekistan; rivuzb@gmail.com; http://orcid.org/0000-0001-5124-4353
Evgeniya I. Kazakova, Cand. Med. Sci., Junior Researcher, Research Institute of Virology, Tashkent, Uzbekistan; rivuzb@gmail.com; http://orcid.org/0000-0003-1265-7299
Nargiz S. Ibadullaeva, Cand. Med. Sci., Senior Researcher, Research Institute of Virology, Tashkent, Uzbekistan; rivuzb@gmail.com; http://orcid.org/0000-0001-8334-2548
Sabina S. Ibragimova, Laboratory Doctor, Republican Center for AIDS Control, Baku, Azerbaijan; cascabell@mail.ru
Aygun M. Safarova, Laboratory Doctor, Republican Center for AIDS Control, Baku, Azerbaijan; aygun.seferova88@gmail.com
Agigat Kadirova, Director, Research Institute of Pulmonary Diseases, Baku, Azerbaijan; office@aids.az
Zukhra Nurlyaminova, Deputy Director, Republican Center for AIDS Prevention and Control, Dushanbe, Tajikistan; zuha_69@bk.ru
Lailo Ismatova, Head, Reference Laboratory, Republican Center for AIDS Prevention and Control, Dushanbe, Tajikistan; l-ismatova@mail.ru
Mohira Zukhurova, Laboratory Doctor, Republican Center for AIDS Prevention and Control, Dushanbe, Tajikistan; mz-87-87@mail.ru
Ramshed Kholnazarov, Infectiologist, Republican Center for AIDS Prevention and Control, Dushanbe, Tajikistan: rholnazarov@mail.ru
Aibek A. Bekbolotov, Deputy Director, Republican AIDS Center, Ministry of Health of the Kyrgyz Republic, Bishkek, Kyrgyzstan; aibek_0001@mail.ru; http://orcid.org/0000-0001-9931-3311
Elmira B. Narmatova, Chief Physician, Osh Regional AIDS Center, Ministry of Health of the Kyrgyz Republic,Osh, Kyrgyzstan; elmira.narmatova@yandex.ru; http://orcid.org/0000-0001-9263-0525
Baarinisa M. Iskanova, Laboratory Assistant, Republican AIDS Center, Ministry of Health of Kyrgyz Republic, Bishkek, Kyrgyzstan; i.barnisa@hotmail.com; http://orcid.org/0000-0002-2493-1788
Trdat R. Grigoryan, Monitoring and Evaluation Specialist, Republican Center for AIDS Prevention, Ministry of Health of the Republic of Armenia, Yerevan, Armenia; gtrdat@yahoo.com; http://orcid.org/0000-0002-7319-7444
Arshak R. Petrosyan, Laboratory Doctor, Laboratory of PCR, Microbiology, Virology and Genetics, Republican Center for AIDS Prevention, Ministry of Health of the Republic of Armenia, Yerevan, Armenia; a_petrosyan@list.ru
Tatevik A. Sargatyan, Laboratory Doctor, Laboratories of PCR, Microbiology, Virology, and Genetics, Republican Center for AIDS Prevention, Ministry of Health of the Republic of Armenia, Yerevan, Armenia; sarhatyantatev@mail.ru
Elena L. Gasich, Cand. Biol. Sci., Associate Professor, Head, Laboratory, Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, Belarus; elena.gasich@gmail.com; http://orcid.org/0000-0002-3662-3045
Anastasia S. Bunas, Junior Researcher, Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, Belarus; rrpcem@belriem.by; http://orcid.org/0000-0001-8641-0104
Iryna N. Glinskaya, Epidemiologist, Republican Center for Hygiene, Epidemiology and Public Health, Minsk, Belarus; mail@rcheph.by; http://orcid.org/0000-0002-3941-9787
Professor Vadim V. Pokrovsky, Academician of the Russian Academy of Sciences, MD, Head, HIV Department, Central Research Institute of Epidemiology, Russian Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being, Moscow, Russia; pokrovsky.vad@yandex.ru; https://orcid.org/0000-0002-9514-7288; Scopus Author ID: 7202457043
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