Molecular epidemiological analysis of HIV-1 genetic variants circulating in the countries of Eastern European and Central Asia in 2010-2019


DOI: https://dx.doi.org/10.18565/epidem.2022.12.3.31-40

Lapovok I.A., Kirichenko A.A., Shlykova A.V., Saleeva D.V., Lopatukhin A.E., Kireev D.E., Ladnaia N.N., Musabaev E.I., Kazakova E.I., Ibadullaeva N.S., Ibragimova S.S., Safarova A.M., Kadirova A., Nurlyaminova Z., Ismatova L., Zukhurova M., Kholnazarov R., Bekbolotov A.A., Narmatova E.B., Iskanova B.M., Grigoryan T.R., Petrosyan A.R., Sargatyan T.A., Gasich E.L., Bunas A.S., Glinskaya I.N., Pokrovsky V.V.

1) Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Right Protection and Human Well-Being, Moscow, Russia; 2) Research Institute of Virology, Tashkent, Uzbekistan; 3) Republic Center for AIDS Control, Baku, Azerbaijan; 4) Research Institute of Pulmonary Diseases, Baku, Azerbaijan; 5) Republican Center for AIDS Prevention and Control, Dushanbe, Tajikistan; 6) Republican AIDS Center, Ministry of Health of the Kyrgyz Republic, Bishkek, Kyrgyzia; 7) Osh Regional AIDS Center, Ministry of Health of the Kyrgyz Republic, Osh, Kyrgyzia; 8) National Center for AIDS Prevention, Yerevan, Armenia; 8) Republican Center for AIDS Prevention, Ministry of Health of the Republic of Armenia, Yerevan, Armenia; 9) Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, Belarus; 10) Republican Center for Hygiene, Epidemiology, and Public Health, Minsk, Belarus
Objective. To characterize HIV-1 genetic variants circulating in the countries of Eastern Europe and Central Asia (EECA) in 2010–2019.
Materials and methods. Nucleotide sequences of the HIV-1 pol gene fragment (2253–3344 bp) in the plasma samples were obtained from HIV-infected patients in Russia (RU), Armenia (AM), Azerbaijan (AZ), Belarus (BY), Kyrgyzstan (KG), Tajikistan (TJ), and Uzbekistan (UZ). The collection was supplemented with nucleotide sequences from the international database.
Results. A total of 1825 nucleotide sequences from the countries studied were analyzed. Infection through unprotected heterosexual contact was the dominant route of transmission (about 50%). There was a decrease in the proportion of injecting drug users in 2010–2019. The dominant HIV-1 genetic variants were subtype A6 (64.82%), CRF63_02A1 (22.58%), and subtype B (7.40%). Moreover, the share of CRF63_02A1 in Central Asian countries was significantly higher than that in other EECA countries. There were 48 unique recombinant forms, 38 of which were AG recombinants. 140 molecular clusters were formed containing 369 (20.22%) sequences.
Conclusion. There is a need for further investigations of HIV-1 genetic diversity in the EECA region and for supervision over the generation and spread of new HIV-1 recombinant forms.

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About the Autors


Ilya A. Lapovok, Cand. Biol. Sci., Senior Researcher, Central Research Institute of Epidemiology, Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; i_lapovok@mail.ru; http://orcid.org/0000-0002-6328-1415
Alina A. Kirichenkо, Researcher, Central Research Institute of Epidemiology, Federal Service for the Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; kotova-kirichenko@mail.ru; http://orcid.org/0000-0002-7116-0138
Anastasia V. Shlykova, Researcher, Central Research Institute of Epidemiology, Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; kotova-kirichenko@mail.ru; http://orcid.org/0000-0002-1390-8021
Daria V. Saleeva, Researcher, Central Research Institute of Epidemiology, Federal Service for the Supervision of Consumer Rights Protection and Human Well-Being; Researcher, State Research Center – Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Moscow, Russia; http://orcid.org/0000-0002-5870-5594
Alexey E. Lopatukhin, Researcher,, Central Research Institute of Epidemiology, Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; a.lopatukhin@gmail.com; http://orcid.org/0000-0002-2826-699X
Dmitry E. Kireev, Cand. Biol. Sci., Head, Laboratory, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Right Protection and Human Well-Being,, Moscow, Russia; dmitkireev@yandex.ru; http://orcid.org/0000-0002-7896-2379
Natalya N. Ladnaia, Cand. Biol. Sci., Senior Researcher, Central Research Institute of Epidemiology, Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; n.ladnaia@hiv-russia.ru; http://orcid.org/0000-0003-2994-151X
Professor Erkin I. Musabaev, MD, Director, Research Institute of Virology, Tashkent, Uzbekistan; rivuzb@gmail.com; http://orcid.org/0000-0001-5124-4353
Evgeniya I. Kazakova, Cand. Med. Sci., Junior Researcher, Research Institute of Virology, Tashkent, Uzbekistan; rivuzb@gmail.com; http://orcid.org/0000-0003-1265-7299
Nargiz S. Ibadullaeva, Cand. Med. Sci., Senior Researcher, Research Institute of Virology, Tashkent, Uzbekistan; rivuzb@gmail.com; http://orcid.org/0000-0001-8334-2548
Sabina S. Ibragimova, Laboratory Doctor, Republican Center for AIDS Control, Baku, Azerbaijan; cascabell@mail.ru
Aygun M. Safarova, Laboratory Doctor, Republican Center for AIDS Control, Baku, Azerbaijan; aygun.seferova88@gmail.com
Agigat Kadirova, Director, Research Institute of Pulmonary Diseases, Baku, Azerbaijan; office@aids.az
Zukhra Nurlyaminova, Deputy Director, Republican Center for AIDS Prevention and Control, Dushanbe, Tajikistan; zuha_69@bk.ru
Lailo Ismatova, Head, Reference Laboratory, Republican Center for AIDS Prevention and Control, Dushanbe, Tajikistan; l-ismatova@mail.ru
Mohira Zukhurova, Laboratory Doctor, Republican Center for AIDS Prevention and Control, Dushanbe, Tajikistan; mz-87-87@mail.ru
Ramshed Kholnazarov, Infectiologist, Republican Center for AIDS Prevention and Control, Dushanbe, Tajikistan: rholnazarov@mail.ru
Aibek A. Bekbolotov, Deputy Director, Republican AIDS Center, Ministry of Health of the Kyrgyz Republic, Bishkek, Kyrgyzstan; aibek_0001@mail.ru; http://orcid.org/0000-0001-9931-3311
Elmira B. Narmatova, Chief Physician, Osh Regional AIDS Center, Ministry of Health of the Kyrgyz Republic,Osh, Kyrgyzstan; elmira.narmatova@yandex.ru; http://orcid.org/0000-0001-9263-0525
Baarinisa M. Iskanova, Laboratory Assistant, Republican AIDS Center, Ministry of Health of Kyrgyz Republic, Bishkek, Kyrgyzstan; i.barnisa@hotmail.com; http://orcid.org/0000-0002-2493-1788
Trdat R. Grigoryan, Monitoring and Evaluation Specialist, Republican Center for AIDS Prevention, Ministry of Health of the Republic of Armenia, Yerevan, Armenia; gtrdat@yahoo.com; http://orcid.org/0000-0002-7319-7444
Arshak R. Petrosyan, Laboratory Doctor, Laboratory of PCR, Microbiology, Virology and Genetics, Republican Center for AIDS Prevention, Ministry of Health of the Republic of Armenia, Yerevan, Armenia; a_petrosyan@list.ru
Tatevik A. Sargatyan, Laboratory Doctor, Laboratories of PCR, Microbiology, Virology, and Genetics, Republican Center for AIDS Prevention, Ministry of Health of the Republic of Armenia, Yerevan, Armenia; sarhatyantatev@mail.ru
Elena L. Gasich, Cand. Biol. Sci., Associate Professor, Head, Laboratory, Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, Belarus; elena.gasich@gmail.com; http://orcid.org/0000-0002-3662-3045
Anastasia S. Bunas, Junior Researcher, Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, Belarus; rrpcem@belriem.by; http://orcid.org/0000-0001-8641-0104
Iryna N. Glinskaya, Epidemiologist, Republican Center for Hygiene, Epidemiology and Public Health, Minsk, Belarus; mail@rcheph.by; http://orcid.org/0000-0002-3941-9787
Professor Vadim V. Pokrovsky, Academician of the Russian Academy of Sciences, MD, Head, HIV Department, Central Research Institute of Epidemiology, Russian Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being, Moscow, Russia; pokrovsky.vad@yandex.ru; https://orcid.org/0000-0002-9514-7288; Scopus Author ID: 7202457043


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