Русский
Studying the transmission of HIV-1 drug resistance in the Republic of Armenia using bioinformatics methods
DOI: https://dx.doi.org/10.18565/epidem.2021.11.4.53-60
Osadchaya O.A., Eroshkin P.V., Kirichenko A.A., Lapovok I.A., Saleeva D.V., Lopatukhin A.E., Shlykova A.V., Kireev D.E., Grigoryan T.R., Petrosyan A.R., Sarhatyan T.F., Pokrovsky V.V.
1) Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; 2) Republican Center for AIDS Prevention, Yerevan, Republic of Armenia
Materials and methods. The authors analyzed 546 nucleotide sequences of the pol gene fragment isolated from HIV-infected patients who had no experience in receiving ARVT from the Republic of Armenia. Nucleotide sequences were analyzed for the presence of resistance mutations and the level of DR to ARVDs using the Stanford University database and a CPR tool. Mutations were assessed from the 2009 SDRM sheet according to the standard WHO protocols. HIV subtypes were identified using the Stanford University database and confirmed by phylogenetic analysis.
Results. The prevalence of mutations associated with the predictive DR was 16.3%. The level of HIV transmitted DR was 5.5%, which is classified as moderate by the WHO. The highest DR was found for 2 drugs (NVP, EFV) of the NNRTI class and for 2 drugs (FTC, 3TC) of the NRTI class. Analysis of HIV-1 genetic variants in the Republic of Armenia and in the Russian Federation showed a high genetic similarity to the epidemics occurring in these countries.
Conclusion. DR to ARVDs has been found to increase from 1.5 to 5.5%. The high (3.8%) resistance has been established for EFV that is prescribed as a first-line drug. It is important to conduct epidemiological surveillance of the spread of DR in the region due to the higher patient coverage with ARVT.
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About the Autors
Olga A. Osadchaya, Junior Researcher, Central Research Institute of Epidemiology, Federal Service for the Surveillance on Consumer Rights Protection and Human Well-Being, Moscow, Russia; osadchaya96@yandex.ru; http://orcid.org/0000-0003-1543-5579
Pavel V. Eroshkin, Junior Researcher, Central Research Institute of Epidemiology, Federal Service for the Surveillance on Consumer Rights Protection and Human Well-Being, Moscow, Russia; eroshkinpvl@gmail.com; http://orcid.org/0000-0001-5386-1574
Alina A. Kirichenko, Scientific Researcher, Central Research Institute of Epidemiology, Federal Service for the Surveillance on Consumer Rights Protection and Human Well-Being, Moscow, Russia; kotova-kirichenko@mail.ru; http://orcid.org/0000-0002-7116-0138
Ilya A. Lapovik – Cand. Biol. Sci., Senior Researcher, Central Research Institute of Epidemiology, Federal Service for the Surveillance on Consumer Rights Protection and Human Well-Being, Moscow, Russia; i_lapovok@mail.ru; http://orcid.org/0000-0002-632801415
Daria V. Saleeva, Junior Researcher, Central Research Institute of Epidemiology, Federal Service for the Surveillance on Consumer Rights Protection and Human Well-Being, Moscow, Russia; dasha_saleeva@inbox.ru; http://orcid.org/0000-0002-5870-5594
Alexey E. Lopatukhin, Researcher, Central Research Institute of Epidemiology, Federal Service for the Surveillance on Consumer Rights Protection and Human Well-Being, Moscow, Russia; a.lopatukhin@gmail.com; http://orcid.org/0000-0002-2826-699X
Anastasia V. Shlykova, Researcher,, Central Research Institute of Epidemiology, Federal Service for the Surveillance on Consumer Rights Protection and Human Well-Being, Moscow, Russia; murzakova_a.v@mail.ru; http://orcid.org/0000-0002-1390-8021
Dmitry E. Kireev, Cand. Biol. Sci., Head, Laboratory of Diagnostics and Molecular Epidemiology of HIV Infection, Central Research Institute of Epidemiology, Federal Service for the Surveillance on Consumer Rights Protection and Human Well-Being, Moscow, Russia; dmitkireev@yandex.ru; http://orcid.org/0000-0002-7896-2379
Trdat R. Grigoryan, Monitoring and Evaluation Specialist, Republican Center for Prevention of AIDS, Yerevan, Republic of Armenia; gtrdat@yahoo.com; http://orcid.org/0000-0002-7319-7444
Arshak R. Petrosyan, Laboratory Physician, Laboratory of PCR, Microbiology, Virology, and Genetics, Republican Center for AIDS Prevention, Yerevan, Republic of Armenia; a_petrosyan@list.ru
Tatevik A. Sarhatyan, Laboratory Physician, Laboratory of PCR, Microbiology, Virology and Genetics, Republican Center for AIDS Prevention, Yerevan, Republic of Armenia; sarhatyantatev @mail.ru
Professor Vadim V. Pokrovsky, Academician of the Russian Academy of Sciences,, MD, Head, Specialized Department of Epidemiology and Prevention of AIDS, Central Research Institute of Epidemiology, Russian Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being; pokrovsky.vad@yandex.ru; https://orcid.org/0000-0002-9514-7288; Scopus Author ID: 7202457043
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