Molecular genetic analysis of HIV-1 among prisoners on antiretroviral therapy in the penitentiary facilities of the Sverdlovsk Region


Alimov A.V., Ustyuzhanin A.V., No­vo­se­lov A.V., Demchuk N.S., Sannikova L.B., Kadyrova O. Yu., Pankov A.P.

1 Yekaterinburg Research Institute of Viral Infections, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Yekaterinburg, Russia; 2 Medical Sanitary Unit № 66, Federal Penitentiary Service, Yekaterinburg, Russia
Objective. To carry out a molecular genetic analysis of HIV-1 among prisoners receiving antiretroviral therapy (ART) in the penitentiary facilities.
Materials and methods. Viral load was determined in 89 prisoners; out of them 32 individuals with a viral load of >1000 copies/ml were examined for HIV-1 resistance to reverse transcriptase inhibitors and protease inhibitors by a genotypic method. A survey method was used to estimate the degree of compliance with antiretroviral therapy in 138 prisoners.
Results. 20 (62.5%) of the 32 examined HIV-1 nucleotide sequences were interpreted as susceptible to 19 antiretroviral drugs. None of the 32 sequences contained mutations of high-level resistance to the prescribed drugs. Genotyping revealed that subtype AFSU was predominant; five genetic variants were CRF03_AB and two variants were CRF63_02A1.
Conclusion. The high or multiple resistance of HIV-1 to reverse transcriptase inhibitors and protease inhibitors is not responsible for the virologic failure of ART in prisoners.
Keywords: phylogenetic analysis, HIV-1, genotyping, drug resistance
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Literature

  1. Trotsenko O.E., Bogach V.V., Kolganov A.V., Starostina I.S., Ivanov A.N., Shamshina E.M., Kurnyavka P.A., Varnavskaya L.S. [Characterization of the epidemic process of HIV infection in the penitentiary system establishments]. Dal'nevostochnyj zhurnal infektsionnoj patologii 2004; (5): 82–7. (In Russ.).
  2. Gromov K.B., Kazennova E.V., Kireev D.E., Lapovok I.A., Lopatuhin A.E., Murzakova A.V., Bobkova M.R. [Genome analysis of the AFSU variant dominating at Russia territory. In: Proceedings of the XI all-Russian scientific-practical conference with international participation «Molecular diagnostics–2017». Pokrovskiy V. I. ed. Vol. 1.]. Tambov: Yulis, 2017; 448–9. (In Russ.).
  3. Wang D., Hicks C.B., Goswami N.D., Tafoya E., Ribeiro R.M., Cai F., Perelson A.S., Gao F. Evolution of drug-resistant viral populations during interruption of antiretroviral therapy. J. Virol. 2011, 85(13): 6403–15. DOI:10.1128/JVI.02389-10.
  4. Pontali E., Ventura A., Bruzzone B., Icardi G., Ferrari F. Unexpected high rate of wild-type HIV-1 genotype among inmates failing antiretroviral therapy. HIV Clin Trials. 2008, 9(5): 341–7. DOI: 10.1310/hct0905-341.
  5. Basson A.E., Rhee S.Y., Parry C.M., El-Khatib Z., Charalambous S., De Oliveira T., Pillay D., Hoffmann C., Katzenstein D., Shafer R.W., Morris L. Impact of drug resistance-associated amino acid changes in HIV-1 subtype C on susceptibility to newer nonnucleoside reverse transcriptase inhibitors. Antimicrob. Agents Chemother. 2015, 59: 960–71. DOI:10.1128/AAC.04215-14.
  6. Baryshev P., Bogachev V., Gashnikova N. HIV-1 genetic diversity in Russia: CRF63_02A1, a new HIV type 1 genetic variant spreading in Siberia. AIDS Res. Hum. Retroviruses 2014, 30(6): 592–7. DOI:10.1089/aid.2013.0196.
  7. Totmenin A.V., Astahova E.M., Ivlev V.V., Zyryanova D.P., Gashnikova M.P., Vlasov E.V., Murankina V.R., Gashnikova N.M. [HIV-1 genotyping specifics with regard to progression of modern territorial epidemics. In: Proceedings of the XI all-Russian scientific-practical conference with international participation «Molecular diagnostics–2017». Pokrovskiy V. I. ed. Vol. 1.]. Tambov: Yulis, 2017; 477–8. (In Russ.).


About the Autors

For correspondence:
Alexey V. Novoselov, Cand. Med. Sci., Head, Urals Federal District Center for AIDS Prevention and Control, Yekaterinburg Research Institute of Viral Infections, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Address: 23, Letnyaya St., Yekaterinburg 620030, Russia
Telephone: +7 (343) 261-99-54
E-mail: ufoaids@mail.ru


Information about the authors:
Aleksandr V. Alimov, Cand. Med. Sci.; Director, Yekaterinburg Research Institute of Viral Infections, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Yekaterinburg, Russia; e-mail: virus@ eniivi.ru
Aleksandr V. Ustyuzhanin, Head, Laboratory, Urals Federal District Center for AIDS Prevention and Control, Yekaterinburg Research Institute of Viral Infections, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Yekaterinburg, Russia; e-mail: ust103@yandex.ru
Natalia S. Demchuk, Biologist, Laboratory, Urals Federal District Center for AIDS Prevention and Control, Yekaterinburg Research Institute of Viral Infections, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Yekaterinburg, Russia; e-mail: demchuk.natalia.s@yandex.ru
Lyubov B. Sannikova, Biologist, Laboratory, Urals Federal District Center for AIDS Prevention and Control, Yekaterinburg Research Institute of Viral Infections, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Yekaterinburg, Russia; e-mail: Sannickova.lub@yandex.ru
Olga Yu. Kadyrova, Lieutenant Colonel, Internal Military Service, Deputy Head for Organizational, Methodological, and Clinical Expert Work, Medical Sanitary Unit № 66, Federal Penitentiary Service, Yekaterinburg, Russia; e-mail: inf_msch66@mail.ru
Aleksandr P. Pankov, Colonel, Internal Military Service; Head, Medical Sanitary Unit № 66, Federal Penitentiary Service, Yekaterinburg, Russia; e-mail: inf_msch66@mail.ru


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