Local cytokine profile in adult patients with acute enteric infections and its correction with immunomodulatory therapy


Buslenko A.O., Aleshukina A.V., Pshenich­naya N Yu., Khukazova S.A., Kostenko O.D., Gopatsa G.V., Malysheva M.I.

1 Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Protection and Human Welfare, Rostov-on-Don, Russia; 2 Rostov State Medical University, Ministry of Health of Russia, Rostov-on-Don, Russia; 3 Center for Prevention and Control of AIDS and Infectious Diseases of the Rostov Region, Rostov-on-Don, Russia
Objective. To assess the intestinal cytokine profile in patients with acute enteric infections (AEI) in the dynamics of the disease during standard pathogenetic therapy and treatment according to the regimen added by aminodihydrophthalazinedione sodium (ADPNа).
Subjects and methods. The patients were divided into 2 groups of 30 people each according to a therapy regimen. Group 1 received standard pathogenetic therapy. In Group 2, the regimen was added by ADPNa. The coprofiltrates taken at 1-2 days of the disease and 5 days later were used to determine the level of cytokines (interferon-γ (IFN-γ), IL-4, IL-6, and IL-10).
Results. In both groups, the levels of IL-6 and IFN-γ substantially increased, but those of IL-4 and IL-10 kept normal in the height of the disease. In Group 2, the level of IL-6 significantly decreased and that of IL-10 increased at hospital discharge. Only the similar insignificant trend was observed in Group 1. Changes in the cytokine profile also affected the duration of the symptoms of the disease. In Groups 1 and 2, the duration of diarrhea was 5.5 ± 0.2 and 4.0 ± 0.2 days, respectively (p < 0.01).
Conclusion. The findings make it possible to recommend that the local cytokine profile should be corrected with ADPNа in the combination treatment of AEI.
Keywords: aminodihydrophthalazinedione sodium, Galavit, acute enteric infections, immunomodulators, cytokines
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About the Autors

For correspondence:
Prof. Natalia Yu. Pshenichnaya, MD; Head, Department of Infectious Diseases with Course of Childhood Infectious Diseases, Faculty for Advanced Training and Professional Retraining of Specialists, Rostov State Medical University, Ministry of Health of Russia; Physician, Clinic of Infectious and Parasitic Diseases, Rostov Research Scientific Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Protection and Human Welfare, Rostov-on-Don,
Address: 29, Nakhichevansky Lane, Rostov-on-Don 344022, Russia
Telephone: +7(863) 232-08-17
E-mail: natalia-pshenichnaya@yandex.ru
Information about the authors:
Asya O. Buslenko, Postgraduate Student, Department of Infectious Diseases with Course of Childhood Infectious Diseases, Faculty for Advanced Training and Professional Retraining of Specialists, Rostov State Medical University, Ministry of Health of Russia; Junior Researcher, Laboratory of Bacteriology and Virology, Rostov Research Scientific Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Protection and Human Welfare, Rostov-on-Don, Russia; e-mail: doctor-futur2007@yandex.ru
Anna V. Aleshukina, MD, Senior Researcher, Head, Bacteriological Laboratory, Rostov Research Scientific Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Protection and Human Welfare, Rostov-on-Don, Russia; e-mail: aaleshukina@mail.ru
Svetlana A. Кhukasova, Junior Researcher, Bacteriological Laboratory, Rostov Research Scientific Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Protection and Human Welfare, Rostov-on-Don, Russia; e-mail: rniimp@yandex.ru
Olga D. Kostenko, Junior Researcher, Bacteriological Laboratory, Rostov Research Scientific Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Protection and Human Welfare, Rostov-on-Don, Russia; e-mail: rniimp@yandex.ru
Galina V. Gopatsa, Cand. Med. Sci., Associate Professor, Department of Infectious Diseases with Course of Childhood Infectious Diseases, Faculty for Advanced Training and Professional Retraining of Specialists, Rostov State Medical University, Ministry of Health of Russia, Rostov-on-Don, Russia; e-mail: galentinabk@mail.ru
Marina I. Malysheva, Chair, Department of Infectious Diseases; Center for prevention and control of AIDS and infectious diseases of the Rostov Region, Rostov-on-Don, Russia; e-mail: doctor-futur2007@yandex.ru


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