Русский
The first cases of Borrelia miyamotoi disease in the Far East of Russia
Leonova G.N., Bondarenko E.I., Ivanis V.A., Belikov S.I., Lubova V.A.
1G.P. Somov Research Institute of Epidemiology and Microbiology, Vladivostok, Russia; 2«Vector-Best», Novosibirsk, Russia; 3Pacific State Medical University, Vladivostok, Russia; 4Limnological Institute, Siberian Branch, Russian Academy of Sciences, Irkutsk, Russia
Objective. To verify Borrelia miyamotoi in Ixodes ticks and in the blood of patients and to describe cases.
Materials and methods. In 2014–2015, the investigators studied 238 I. persulcatus samples collected from vegetation; 253 I. persulcatus samples taken from patients; and 168 leukocyte fraction samples from patients after tick suction. Real-time PCR was performed using MultiPrime reagent kits for the amplification of cDNA fragments of tick-borne encephalitis virus, B. burgdorferi s.I, A. phagocytophilum, E. chaffeensis/E. muris-FL (Central Research Institute of Epidemiology, Moscow), and B. miyamotoi («Vector-Best», Novosibirsk). The B. miyamotoi glpQ regions with a length of 680 bp were sequenced.
Results. The typical symptoms of the disease caused by B. miyamotoi were detected in only two out of 21 verified patients (14 I. persulcatus specimens taken from the patients and 7 leukocyte fraction samples from the patients after tick suction). Sequencing of the glpQ gene and phylogenetic analysis showed genetic differences between B. miyamotoi and B. burgdorferi s.I. and a similarity with the pathogens of a relapsing fever Borrelia group.
Conclusion. Among the tick-borne pathogens, not only B. burgdorferi s.l. causing Lyme disease, but also the new pathogen B. miyamotoi previously unknown in the Far East plays a great role.
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About the Autors
For correspondence:
Prof. Galina N. Leonova, MD, Principal Researcher, G.P. Somov Research Institute of Epidemiology and Microbiology
Address: 1 Selskaya St., Vladivostok 690087, Russia
Telephon: +7(423) 244-07-12
E-mail: galinaleon41@gmail.com
Information about the authors:
Еvgeni I. Bondarenko, Cand. Biol. Sci., Researcher, AO «Vector-Best», Novosibirsk, Russia; е-mail: ebondarenko@ngs.ru
Viktoria A. Ivanis, MD, Professor, Department of Infectious Diseases, Pacific State Medical University, Vladivostok, Russia; е-mail: ivanis2003@bk.ru
Prof. Sergei I. Belikov, BD, Head of Laboratory, Limnological Institute, Siberian Branchof the Russian Academy of Sciences, Irkutsk, Russia; e-mail: sergeibelikov47@gmail.com
Viktoria A. Lubova, Junior Researcher, G.P. Somov Research Institute of Epidemiology and Microbiology, Vladivostok, Russia; e-mail: valeri_priority@mail.ru
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