Characteristics of Brucella melitensis strains isolated in different regions of the Russian Federation


DOI: https://dx.doi.org/10.18565/epidem.2024.14.4.70-6

Serdyuk N.S., Zhilchenko E.B., Kuznetsova I.V., Zharinova N.V., Kovalev D.A., Shapakov N.A., Khachaturova A.A., Belozerova O.N., Karapetyan M.G.

Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia
Objective. Evaluation of the phenotypic and molecular genetic properties of B. melitensis strains isolated in brucellosis-affected territories of the Russian Federation from 1949 to 2019.
Materials and methods. We used 74 strains of the causative agent of brucellosis from the collection of the Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being,and 3 reference strains (63/9, 16M and 706) from the MLVA database http://microbesgenotyping.i2bc.paris-saclay.fr/databases.
Results. The studied strains of B. melitensis were distinguished by significant diversity in 14 VNTR loci. It was revealed that the strains of B. melitensis were divided into two large clusters. One cluster is formed by strains circulating in the Asian part of Russia, and the second cluster is represented by strains from the European part of Russia - the North Caucasian Federal District (NCFD) and the Southern Federal District (SFD).
Conclusion. The obtained data are used for continuous molecular genetic monitoring of the causative agent of brucellosis in the territories of Russia unfavorable for brucellosis, including as an effective tool for retrospective epidemiological analysis, which will allow to qualitatively improve the epidemiological surveillance of this infection. The use of complex data of both phenotypic and genetic nature allows to expand the possibilities of differentiation of strains during the investigation of brucellosis outbreaks in the future.

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About the Autors


Natalia S. Serdyuk, Cand. Biol. Sci., Senior Researcher, Laboratory of Collection of Pathogenic Microorganisms, Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; nata.serdyuk.83@mail.ru; http://orcid.org/0000-0001-5350-0111
Elena B. Zhilchenko, Cand. Biol. Sci., Head, Laboratory of Collection of Pathogenic Microorganisms, Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; stavnipchi@mail.ru; https://orcid.org/0000-0001-7180-1292
Irina V. Kuznetsova, Cand. Med. Sci., Senior Researcher, Biochemistry Laboratory, Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; stavnipchi@mail.ru; https://orcid.org/0000-0001-9513-0761
Nina V. Zharinova, Cand. Biol. Sci., Senior Researcher, Pathogenic Microorganisms Collection Laboratory, Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; stavnipchi@mail.ru; https://orcid.org/0000-0001-6718-4877
Dmitry A. Kovalev, Cand. Chem. Sci., Head., Biochemistry Laboratory, Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; stavnipchi@mail.ru; https://orcid.org/0000-0002-9366-5647
Nikolay A. Shapakov, Junior Researcher, Biochemistry Laboratory, Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; stavnipchi@mail.ru; https://orcid.org/0000-0001-9152-4026
Anna A. Khachaturova, Researcher, Brucellosis Laboratory, Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; stavnipchi@mail.ru; https://orcid.org/0000-0002-7459-7204
Olga N. Belozerova, Junior Researcher, Laboratory of Collection of Pathogenic Microorganisms, Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; stavnipchi@mail.ru; https://orcid.org/0009-0002-5570-557X
Manya G. Karapetyan. Junior Researcher, Laboratory of Collection of Pathogenic Microorganisms, Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; stavnipchi@mail.ru; https://orcid.org/0009-0005-3006-5504


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