Русский
Genetic typing of Brucella melitensis strains on the basis of variability analysis of INDEL loci
DOI: https://dx.doi.org/10.18565/epidem.2022.12.1.81-6
Kovalev D.A., Kuznetsova I.V., Zhirov A.M., Serdyuk N.S., Zhilchenko E.B., Ponomarenko D.G., Vodopyanov A.S., Vodopyanov S.O., Kulichenko A.N.
1) Stavropol Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; 2) Rostov-on-Don Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia
Materials and methods. Molecular genetic typing of 11 B. melitensis strains was carried out by polymerase chain reaction with electrophoretic detection of results. Forty-eight B. melitensis strains present in the GenBank international database were genotyped in silico.
Results. Five INDEL genotypes were identified among the 59 studied strains. Based on cluster analysis, all genotypes were grouped into 5 clusters. A correlation was established between the INDEL genotype of B. melitensis strains and their belonging to one of the 5 main genetic lines of the microorganism based on whole genome SNP analysis (wgSNP).
Conclusion. The findings allow us to consider the developed INDEL typing method as a promising additional tool for defining the origin of individual B. melitensis isolates during epidemiological investigations of brucellosis outbreaks.
Literature
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About the Autors
Dmitry A. Kovalev, Cand. Med. Sci., Head, Laboratory of Biochemistry, Stavropol Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; kovalev_da.stv@list.ru; https://orcid.org/0000-0002-9366-5647
Irina V. Kuznezova, Bacteriologist, Laboratory of Biochemistry, Stavropol Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being Stavropol, Russia; stavnipchi@mail.ru; http://orcid.org/0000-0001-9513-0761
Andrew M. Zhirov, Researcher, Laboratory of Biochemistry, Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being Stavropol, Russia; stavnipchi@mail.ru; http://orcid.org/0000-0002-7698-7361
Natalia S. Serdyuk, Cand. Biol. Sci., Biologist, Laboratory of Collection of Pathogenic Microorganisms, Stavropol Antiplague Institute Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; stavnipchi@mail.ru
Elena B. Zhilchenko, Cand. Biol. Sci., Head, Laboratory of Collection of Pathogenic Microorganisms, Stavropol Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; stavnipchi@mail.ru
Dmitry G. Ponomarenko, Cand. Biol. Sci., Head, Laboratory of Brucellosis, Stavropol Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; stavnipchi@mail.ru
Alexey S. Vodop’janov, Cand. Med. Sci., Head, Group of Virology, Rostov-on-Don Research Antiplague Institute, Rostov-on-Don, Russia; alexvod@gmail.com,; https://orcid.org/0000-0002-9056-3231.
Sergey O. Vodopyanov, MD, Head, Laboratory of Microbial Biochemistry, Rostov-on-Don Antiplague Institute Rostov-on-Don, Russia; serge100v@gmail.com; https://orcid.org/0000-0003-4336-0439
Professor Aleksandr N. Kulichenko, MD, Corresponding Member of the Russian Academy of Sciences; Director, Stavropol Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol, Russia; stavnipchi@mail.ru; https://orcid.org/0000-0002-9362-3949
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