Formation of specific T-cell and humoral immunity in unvaccinated and vaccinated children with pertussis


DOI: https://dx.doi.org/10.18565/epidem.2023.14.1.36-42

Fedorova I.M., Popova O.P., Blyakher M.S., Koteleva S.I., Skirda T.A., Kapustin I.V., Ramazanovа Z.K., Tulskaya E.A., Sandalova S. .V., Odintsov E.E., Shvetsova Yu.V.

1) G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology of Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; 2) Russian Medical Academy of Continuous Professional Education, Ministry of Health of Russia, Moscow, Russia; 3) Infectious Clinical Hospital № 1 of the Moscow Healthcare Department, Moscow, Russia
Objective. Evaluation of the relationship between the formation of antigen (AG) – specific T-cell and humoral immunity and its cytokine regulation in the dynamics of pertussis in children.
Materials and methods. 100 children with pertussis were examined at 2–5 weeks of disease and during the period of convalescence. The balance of IFN-γ and IL-4 cytokines in the blood was compared with the dynamics of the specific immune response to B. pertussis antigens. The humoral response was assessed by enzyme immunoassay using Ridascreen test systems, and the T-cell response was assessed by the proliferation of lymphocytes isolated from the blood of patients after incubation on plates from the same test systems.
Results. In 69–92% of unvaccinated children, antigen-specific T-cells are detected from the 2nd to 12th week from the onset of the disease with a temporary sharp decrease in indicators at the 5–6th week. In vaccinated children with pertussis, the immune response develops as a secondary one, but memory T cells are found in no more than 67% of patients.
Conclusion. The nature of cytokine regulation of antibody formation in the dynamics of pertussis is reflected in the process of formation of memory T-cells and depends on the vaccine history.

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About the Autors


Irina M. Fedorova, Cand. Med. Sci., Leading Researcher, Department of Immunology, G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; vestnik-07@mail.ru; http://orcid.org/0000-0002-0335-2752
Olga P. Popova, MD, Professor, Department of Children’s Infectious Diseases, Russian Medical Academy of Continuous Professional Education, Ministry of Health of Russia; Leading Researcher, Department of Immunology, G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; doctorpopova@yandex.ru; http://orcid.org/0000-0002-1772-5978
Professor Maria S. Blyakher, MD, Head, Laboratory of the Immunology Department, G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; maria.s.b@bk.ru; http://orcid.org/0000-0003-3480-6873
Svetlana I. Koteleva, Cand. Med. Sci., Leading Researcher, Department of Immunology, G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; felileo@yandex.ru; http://orcid.org/0000-0003-1878-2234
Tatyana A. Skirda, Cand. Med. Sci., Leading Researcher, Laboratory of Coccal Infections of the Epidemiological Department, G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; t.skirda@mail.ru; http://orcid.org/0000-0003-4140-1014
Ivan V. Kapustin, Cand. Med. Sci., Leading Researcher, Department of Immunology, G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; vano-kapusta@mail.ru; http://orcid.org/0000-0001-6191-260X
Zarema K. Ramazanova, Cand. Med. Sci, Leading Researcher, Department of Immunology, G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; rzarema@mail.ru; http://orcid.org/0000-0002-9314-3312
Elena A. Tulskaya, Cand. Biol. Sci., Leading Researcher, Department of Immunology, G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; etul@mail.ru; http://orcid.org/0000-0003-1969-4009
Svetlana V. Sandalova, Researcher, Department of Immunology, G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; s.sandalova74@mail.ru; http://orcid.org/0000-0002-4548-9888
Evgeniy E. Odintsov, Cand. Med. Sci., Senior Researcher, Department of Immunology, G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; evgenyodin@yahoo.com; http://orcid.org/0000-0001-5895-2520
Juliya V. Shvetsova, Infectious Disease Physician, Pediatrician of the 3rd Pediatric Department, Infectious Clinical Hospital № 1, Moscow Healthcare Department, Moscow, Russia; juliashvetsova1983@gmail.com


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