Русский
Screening for STI pathogens in vulnerable groups outside of healthcaare facilities: anal canal self-swabbing
DOI: https://dx.doi.org/10.18565/epidem.2022.12.3.46-53
Popova A.A., Domonova E.A., Goloveshkina E.N., Gromova A.V., Makhova T.I., Petrov A.S., Korenev D.A., Barsky K.A., Pokrovskaya A.V.
1) Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; 2) “Shagi” (Steps) Regional Charitable Public AIDS Control Foundation, Moscow, Russia; 3) Peoples’ Friendship University of Russia, Moscow, Russia
Subjects and methods. The investigation involved 251 people. (88.4% were men) living in Moscow and the Moscow Region. All the investigation participants took independently their biological material from the anal canal, by using an individual kit of consumables (Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being). The ease of using the kit was assessed using a questionnaire. The DNA of STI pathogens (N. gonorrhoeae, C. trachomatis, M. genitalium, T. vaginalis, and T. pallidum) was detected by real-time polymerase chain reaction with fluorescence signal detection.
Results. In 23.5% of the participants, the anal canal mucosal samples exhibited DNA of N. gonorrhoeae [42.4% (25/59)], C. trachomatis [37.3% (22/59)], M. genitalium [30.5% (18/59)], and T. pallidum [3.4% (2/59)]. The survey results showed that the investigation participants rated the ease of taking biological material at an average score of 9.4 (difficult = 0, easy = 10), the availability of understanding and the ease of following the instructions at an average score of 9.7 (difficult = 0, easy = 10), ease of use at an average score of 8.9 (very uncomfortable = 0, very comfortable = 10). An assessment of the personal preferences of the patents in choosing a method for taking biological material indicated that only 4.7% of the respondents would prefer traditional visits to their physician in the future; 37.1% would prefer the use of a tested approach and 56.6% would not exclude both methods.
Conclusion. Testing the anal canal mucosal samples showed that the prevalence of STI pathogens (N. gonorrhoeae, C. trachomatis, M. genitalium, T. vaginalis, and T. pallidum) among the representatives of vulnerable groups in the Moscow Region was 23.5%. The investigation participants rated the individual kit of consumables as comfortable and easy to use, the use instructions for use as easy to understand. 93.7% of respondents considered that the personalized kit was the best fit for their application, whereas 37.1% would choose the kit rather than their traditional doctor’s visit. The use of a kit for anal canal biological material self-sampling for PCR testing will be able to significantly increase the coverage of the vulnerable population as part of screening for STI pathogens.
Literature
1. Кубанов А.А., Богданова Е.В. Итоги деятельности медицинских организаций, оказывающих медицинскую помощь по профилю дерматовенерология, в 2020 году: работа в условиях пандемии. Вестник дерматологии и венерологии 2021; 97(4): 8–32. doi: 10.25208/vdv1261 Kubanov A.A., Bogdanova E.V. [Dermatovenereology of Russian Federation in 2020: Working Under a Pandemic].Bulletin of Dermatology and Venereology 2021; 97(4): 8–32. (In Russ.). doi: 10.25208/vdv1261 2. Попова А.А., Покровская А.В., Шедько Е.Д., Громова А.В., Скачкова Т.С., Головешкина Е.Н. и др. Частота выявления инфекций, передаваемых половым путем, по результатам скрининга трех анатомических локусов у пациентов из групп риска. Эпидемиол. инфекц. болезни. Актуал. вопр. 2021; 11(4): 73–9. doi: 10.18565/epidem.2021.11.4.73-9 Popova A.A., Pokrovskaya A.V., Shedko E.D., Gromova A.V., Skachkova T.S., Goloveshkina E.N. et al. [Detection rate for sexually transmitted infections by screening for three anatomic loci in at-risk patients]. Epidemiology and infectious diseases. Current items 2021; 11(4): 73–9. (In Russ.). doi: 10.18565/epidem.2021.11.4.73-9 3. Махова Т.И., Громова А.В., Скачкова Т.С., Шедько Е.Д., Головешкина Е.Н., Попова А.А. и др. Частота выявления различных нарушений микробиоты влагалища и возбудителей инфекций, передаваемых половым путем, у женщин из группы риска. Проблемы медицинской микологии 2021; 23(2): 112. Makhova T.I., Gromova A.V., Skachkova T.S., Shedko E.D., Goloveshkina E.N., Popova A.A. [Prevalence of various disorders of the vaginal microbiota and STI among women sex-workers]. Problems in Medical Mycology 2021; 23(2): 112. (In Russ.). 4. Тюленев Ю.А., Гущин А.Е., Кисина В.И. Особенности локализации инфекций, передаваемых половым путем, у субъективно асимптомных мужчин, практикующих секс с мужчинами. Клиническая дерматология и венерология 2021; 20(1): 55–61. doi: 10.17116/klinderma20212001155 Tyulenev Yu.A., Guschin A.E., Kisina V.I. [Localization features of sexually transmitted infections in subjectively asymptomatic men who have sex with men]. Clinical Dermatology and Venereology 2021; 20(1): 55–61. (In Russ.). doi:10. 17116/klinderma20212001155 5. Тюленев Ю.А., Титов И.С., Гущин А.Е. Высокая частота инфекций, передаваемых половым путем, среди мужчин, практикующих секс с мужчинами, обращающихся за проктологической помощью. Клиническая дерматология и венерология 2018; 17(3): 103. doi: 10.17116/klinderma2018173109 Tyulenev Yu.A., Titov I.S., Gushchin A.E. [High incidence of sexually transmitted infections in men who have sex with men and visit proctology clinics]. Сlinical Dermatology and Venerology 2018; 17(3): 103. (In Russ.). doi: 10.17116/klinderma2018173109 6. Khan Z., Bhargava A., Mittal P., Bharti R., Puri P., Khunger N. Evaluation of reliability of self-collected vaginal swabs over physician-collected samples for diagnosis of bacterial vaginosis, candidiasis and trichomoniasis, in a resourcelimited setting: a cross-sectional study in India. BMJ Open. 2019; (9): e025013. doi: 10.1136/bmjopen-2018-025013 7. Lunny C., Taylor D., Hoang L., Wong T., Gilbert M., Lester R. et al. Self-Collected versus Clinician-Collected Sampling for Chlamydia and Gonorrhea Screening: A Systemic Review and Meta-Analysis. PLoS ONE 2015; 10(7): e0132776. doi: 10.1371/journal.pone.0132776 8. Yared N., Horvath K., Fashanu O., Zhao R., Baker J., Kulasingam S. Optimizing Screening for Sexually Transmitted Infections in Men Using Self-Collected Swabs: A Systematic Review, Sexually Transmitted Diseases 2018; 45 (Issue 5): 294–300. doi: 10.1097/OLQ.0000000000000739 9. Bell S., Wapling J., Ase S. Boli-Neo R., Andrew J Vallely, John M Kaldor. Acceptability of testing for anorectal sexually transmitted infections and self-collected anal swabs in female sex workers, men who have sex with men and transgender women in Papua New Guinea. BMC Public Health 2018; 18(1): 776. doi: 10.1186/s12889-018-5684-2 10. Templeton D.J., Wang Y., Higgins A.N. et al. Self-collected anal swabs in men who have sex with men: minimal benefit of routine peri-anal examination. Sexually Transmitted Infections 2011; 87: 204. 11. Попова А.А. Значение использования набора для самостоятельного взятия мазка при скрининге на ИППП у пациентов из групп риска за пределами медицинских учреждений». Материалы VIII Конгресса Евро-Азиатского общества по инфекционным болезням. Москва, 17–19 мая 2022 г. https://eacongress.ru/home Popova A.A. [Importance of using the self-swab kit for screening for STIs in at-risk patients outside of health care settings. Materials VIII Congress of the Euro-Asian Society for Infectious Diseases]. Moscow, May 17–19, 2022. (In Russ.). https://eacongress.ru/home 12. Взятие, транспортировка, хранение биологического материала для ПЦР-диагностики. Методические рекомендации. М.: ФБУН ЦНИИЭ, 2021. 112 с. doi: 10.36233/978-5-6045286-6-2 Collection, transportation, storage of biological material for PCR diagnostics. Methodological recommendations. Moscow: Central Research Institute of Epidemiology, 2021. 112 p. (In Russ.). doi: 10.36233/978-5-6045286-6-2
About the Autors
Anna A. Popova, Cand. Med. Sci., Senior Researcher, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; asya-med@mail.ru; https://orcid.org/0000-0001-9484-5917
Elvira A. Domonova, Cand. Boil. Sci., Head, Scientific Group for Development of New Methods for Diagnosing Opportunistic and Papillomavirus Infections, Department for Molecular Diagnosis and Epidemiology, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; еlvira.domonova @pcr.ms; http://orcid.org/0000-0001-8262-3938
Elena N. Goloveshkina, Сand. Biol. Sci., Head, Laboratory for Molecular Diagnosis and Epidemiology of Reproductive Organ Infections, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; goloveshkina@cmd.su; https://orcid.org/0000-0002-0536-2874
Anastasia V. Gromova, Cand. Vet. Sci., Researcher, Laboratory for Molecular Diagnosis and Epidemiology of Reproductive Organ Infections, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; anastgromow@yandex.ru; https://orcid.org/0000-0003-4664-3178
Tamara I. Makhova, Researcher, Laboratory for Molecular Diagnosis and Epidemiology of Reproductive Organ Infections, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; tamara.makhova89@gmail.com; https://orcid.org/0000-0001-7502-6473
Andreу S. Petrov, Social Work Specialist, Vulnerable Population Specialist, «Shagi» (Steps) Regional Charitable Public AIDS Control Foundation «STEPS», Moscow, Russia; mosnomad@gmail.com
Dmitry A. Korenev, Social Work Specialist, Administrator, «Shagi» (Steps) Social Care Information Center, «Shagi» (Steps) Regional Charitable Public AIDS Control Foundation, Moscow, Russia; 1dmitrii.korenev@gmail.com
Kirill A. Barsky, Public Health and Social Work Specialist; Programs and Projects Manager; «Shagi» (Steps) Regional Charitable Public AIDS Control Foundation, Moscow, Russia; barskiykirill@gmail.com
Anastasia V. Pokrovskaya, Cand. Med. Sci., Senior Researcher, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being; Associate Professor, Department of Infectious Diseases with Courses of Epidemiology and Phthisiology, Medical Institute, Peoples’ Friendship University, Moscow, Russia; pokrovskaya_av@mail.ru; http://orcid.org/0000-0002-2677-0404
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