Genetic characterization of HIV-1 variants circulating in the Republic of Guinea in 2015–2017


DOI: https://dx.doi.org/10.18565/epidem.2019.9.2.44-51

Lapovok I.A., Saleeva D.V., Kirichenko A.A., Murzakova A.V., Lopatukhin A.E., Kireev D.E., Kasyan Zh.A. Agafonov D.A., Nikiforov K.A., Plekhanov N.A., Bayandin R.B., Kartashov M.Yu., Shipovalov A.V., Gashnikova M.P., Shipulin G.A., Boumbaly S., Boiro M.Y.

1) Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; 2) Russian Research Anti-Plague Institute «Microbe», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Saratov, Russia; 3) State Research Center of Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Koltsovo, Novosibirsk Region, Russia; 4) Research Institute of Applied Biology of Guinea, Kindia, Republic of Guinea
Despite the widespread prevalence of HIV infection in Africa, the specific features of its epidemic in a number of countries in this continent have not been sufficiently studied.
Objective. To carry out a molecular biological analysis of HIV variants circulating in the territory of the Republic of Guinea in 2015–2017.
Materials and methods. Plasma samples were collected from 5,566 citizens residing in the Republic of Guinea and were tested for HIV infection. The nucleotide sequences of the pol gene of HIV-1 were studied in HIV-positive samples; the structure and level of drug resistance in the virus were determined.
Results. HIV-1 infection was detected in 200 (3.59%) individuals. No cases of HIV-2 infection were found. The sequences were obtained for 35 samples, 30 (85.71%) of them were AG recombinants, 6 (17.14%) contained nucleoside reverse transcriptase inhibitor (NRTI) and non-NRTI resistance mutations.
Keywords: Republic of Guinea, sequencing, drug resistance, HIV
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Literature

  1. Delatorre E., Be G. Time-scale of minor HIV-1 complex circulating recombinant forms from Central and West Africa. BMC Evol. Biol. 2016; 16(1): 2490. DOI: 10.1186/s12862-016-0824-8
  2. Worobey M., Gemmel M., Teuwen D.E., Haselkorn T., Kunstman K., Bunce M. et al. Direct evidence of extensive diversity of HIV-1 in Kinshasa by 1960. Nature 2008; 455: 661–4. DOI: 10.1038/nature07390.
  3. Faria N.R., Rambaut A., Suchard M.A., Baele G., Bedford T., Ward M.J. et al. The early spread and epidemic ignition of HIV-1 in human populations. Science 2014; 56: 56–61. DOI: 10.1126/science.1256739.
  4. Mir D., Jung M., Delatorre E., Vidal N., Peeters M., Bello G. Phylodynamics of the major HIV-1 CRF02_AG African lineages and its global dissemination. Infect. Genet. Evol. 2016; 46: 190–9. DOI: 10.1016/j.meegid.2016.05.017.
  5. HIV Databases of Los Alamos National Laboratory. https://www. hiv.lanl.gov/content/index
  6. USAID HEALTH PROFILE: Guinea HIV/AIDS Report. 2005. https://web. archive.org/web/20081113214710/http://www.usaid.gov/our_work/global_health/aids/Countries/africa/guinea_05.pdf
  7. UNAIDS. Giunea country factsheets report 2017. http://www.unaids.org/en/ regionscountries/countries/guinea.
  8. Charpentier C., Bellecave P., Cisse M., Mamadou S., Diakite M., Peytavin G. et al. High prevalence of antiretroviral drug resistance among HIV-1-untreated patients in Guinea-Conakry and in Niger. Antivir. Ther. 2011; 16(3): 429–33. DOI: 10.3851/IMP1754.
  9. Freimanis G.L., Loua A., Allain J.P. HIV-1 subtypes D and F are prevalent in Guinea Conakry. J. Clin. Virol. 2012; 53(4): 350–3. DOI: 10.1016/j.jcv. 2011.12.026.
  10. Diouara A.A., Ndiaye H.D., Guindo I., Bangoura N., Cissé M., Edmond T. et al. Antiretroviral treatment outcome in HIV-1-infected patients routinely followed up in capital cities and remote areas of Senegal, Mali and Guinea-Conakry. J. Int. AIDS Soc. 2014; 17: 19315. DOI: 10.7448/IAS.17.1.19315.
  11. Los Alamos HIV database. https://www.hiv.lanl.gov.
  12. Riva C., Romano L., Saladini F., Lai A., Carr J.K., Francisci D. et al. Identification of a possible ancestor of the subtype A1 HIV Type 1 variant circulating in the former Soviet Union. AIDS Res Hum Retroviruses. AIDS Res. Hum. Retroviruses 2008; 24(10): 1319–25. DOI: 10.1089/aid.2008. 0119.
  13. Menéndez-Arias L., Alvarez M. Antiretroviral therapy and drug resistance in human immunodeficiency virus type 2 infection. Antiviral Res. 2014; 102: 70–86. DOI: 10.1016/j.antiviral.2013.12.001.
  14. World Health Organization. Ebola situation report 16 December 2015. http://apps.who.int/iris/bitstream/10665/202501/1/ebolasitrep_16Dec2015_eng.pdf
  15. Leuenberger D., Hebelamou J., Strahm S., De Rekeneire N., Balestre E., Wandeler G. Dabis F; IeDEA West Africa study group. Impact of the Ebola epidemic on general and HIV care in Macenta, Forest Guinea, 2014. AIDS. 2015; 29(14): 1883–7. DOI: 10.1097/QAD.0000000000000784.
  16. Ndawinz J.D., Cissé M., Diallo M.S., Sidibé C.T., D’Ortenzio E. Prevention of HIV spread during the Ebola outbreak in Guinea. Lancet 2015; 385(9976): 1393.
  17. REGA HIV-1 Subtyping Tool – Version 3.0. http://dbpartners.stanford.edu: 8080/RegaSubtyping/stanford-hiv/typingtool/.
  18. COMET HIV-1 v2.2 Subtyping Tool. https://comet.lih.lu.
  19. Stanford University HIV Drug Resistance Database. http://hivdb.stanford.edu.
  20. Major HIV-1 Drug Resistance Mutations. Updated Feb 4, 2019. https://hivdb. stanford.edu/assets/media/resistance-mutation-handout-feb2019.b0204a57. pdf


About the Autors

Ilya A. Lapovok, Cand. Biol. Sci., Researcher, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; е-mail: i_lapovok@mail.ru; ORCID: https://orcid.org/0000-0002-6328-1415
Daria V. Saleeva, Laboratory Assistant-Researcher, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; e-mail: dasha_ saleeva@inbox.ru; ORCID: https://orcid.org/0000-0002-5870-5594
Alina A. Kirichenko, Junior Researcher, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; e-mail: kotova-kirichenko@mail.ru; ORCID: http://orcid.org/0000-0002-7116-0138
Anastasia V. Murzakova, Junior Researcher, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; e-mail: murzakova_ a.v@mail.ru; ORCID: https://orcid.org/0000-0002-1390-8021
Alexey E. Lopatukhin, Researcher, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; e-mail: a.lopatukhin@gmail.com; ORCID: https://orcid.org/0000-0002-2826-699X
Dmitry E. Kireev, Cand. Biol. Sci., Researcher, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; e-mail: dmitkireev@yandex.ru; ORCID: https://orcid.org/0000-0002-7896-2379
Janetta A. Kasyan, Cand. Biol. Sci., Senior Researcher, Departament for Diagnostics of Infectious Disseases, Russian Research Anti-Plague Institute «Microbe», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Saratov, Russia; e-mail: kas.ja2na@rambler.ru; ORCID: http://orcid.org/0000-0001-8230-1672
Dmitry A. Agafonov, Cand. Biol. Sci., Senior Researcher, Department of Microbiology, Russian Research Anti-Plague Institute «Microbe», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Saratov, Russia; e-mail: stone_dragon@mail.ru ORCID: http://orcid.org/0000-0001-9273-6063
Konstantin A. Nikiforov, Cand. Med. Sci, Senior Researcher, Department of Microbiology, Russian Research Anti-Plague Institute «Microbe», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Saratov, Russia; e-mail: nikiforov6666666@mail.ru; ORCID: http://orcid.org/https://orcid.org/0000-0002-4115-9486
Nikita A. Plehanov, Cand. Biol. Sci., Senior Researcher, Department of Microbiology, Russian Research Anti-Plague Institute «Microbe», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Saratov, Russia; e-mail: muscari.sp@yandex.ru; ORCID: http://orcid.org/https://orcid.org/0000-0002-2355-7018
Roman B. Bayandin, Cand. Biol. Sci., Researcher, State Research Center of Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Koltsovo, Novosibirsk Region, Russia; e-mail: romb@ngs.ru; ORCID: http://orcid.org/0000-0002-6460-0828
Mikhail Yu. Kartashov, Cand. Biol. Sci., Senior Researcher, State Research Center of Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Koltsovo, Novosibirsk Region, Russia; e-mail: mikkartash@yandex.ru; ORCID: http://orcid.org/0000-0002-7857-6822
Andrey V. Shipovalov, Researcher, State Research Center of Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Koltsovo, Novosibirsk Region, Russia; e-mail: shipovalov_av@vector.nsc.ru
Maria P. Gashnikova, Laboratory Assistant-Researcher, State Research Center of Virology and Biotechnology «Vector», Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Koltsovo, Novosibirsk Region, Russia; e-mail: gashnikova_ mp@vector. nsc.ru; ORCID: http://orcid.org/0000-0001-9888-5422
German A. Shipulin, Cand. Med. Sci., Head, Departament of Molecular Diagnosti and Epidemiology, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; e-mail: shipgerman@gmail.com; ORCID: http://orcid.org/0000-0002-3668-6601
Boumbaly Sanaba, Cand. Biol. Sci., Director, Medical School, Research Institute of Applied Biology of Guinea, Kindia, Republic of Guinea; e-mail: drboumbaly@yahoo.fr, ORCID: https://orcid.org/0000-0002-4506-6033
Boiro Mamadu Yero, Cand. Biol. Sci., Professor, Director-General, Research Institute of Applied Biology of Guinea, Kindia, Republic of Guinea; e-mail: myboiro@yahoo.fr, ORCID: https://orcid.org/0000-0003-3339-9380


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