Role of current typing methods in investigating the genetic diversity of Salmonella strains isolated from different sources and their antibiotic susceptibility


Rozhnova S.Sh., Guseva A.N., Khristyukhina O.A., Vitkova O.N., Krutova N.E.

1Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare, Moscow, Russia; 2Veterinary Center, Moscow, Russia
Objective. To use different typing methods to characterize Salmonella strains isolated from various sources, including those during salmonellosis outbreaks.
Materials and methods. There were data of a reference center for salmonellosis monitoring on the biological characteristics of 4240 Salmonella strains isolated from humans and animals, foodstuffs and environmental objects in different areas of the country in 2011–2016. Serotyping was performed using Enteroclons monoclonal reagents («SIFIN», Germany). Phage typing of S. enteritidis was carried out applying the collection of type phages, which had been designed by the National Institute of Hygiene (Hungary). Antibiotic susceptibility was determined on the ATBTM G-5 plates («bioMerieux», France), the structure of the strains was ascertained by analysis of a set of total DNA restriction products with pulsed field gel electrophoresis (PFGE).
Results. The leading strains in the etiological structure of salmonellosis were S. enteritidis (39.5 to 71.3%), followed by S. typhimurium (2.0 to 6.7%) and S. infantis (4.8 to 9.0%). 85% of the S. enteritidis strains isolated from different sources were shown to belong to phage type 1. 72% of the strains of this serovar were susceptible to all tested antibiotics. The susceptibility to all antibiotics was revealed in S. typhimurium (19%) and S. infantis (32%). The strains resistant to several antibiotics were predominant among S. infantis strains. Molecular genetic typing has shown that Salmonella strains belonging to the same PFGE pattern, leading of which were JEGX 01.0001–JEGA 26.0001 (S. enteritidis), JPXX 01.0013–JPXA 26.0009 (S. typhimurium), and JFXX 01.0015–JFXA 26.0011 (S. infantis), circulate in different areas of the country.
Conclusion. PFGE analysis was attempted to identify Salmonella strains that are difficult to diagnose.

Literature


1. Directive 2003/99/EC of the Europian parlament and of the Conneil of 17 Nov 2003. Official Journal of the Europian Union. 12.12.2003. L 325/31.

2. Seallan E., Hoekstra R.M., Angulo F.J., Tauxe R.V., Widdowson M.A., Roy S.L., S.L., Jones J.L., Griffin P.M. Foodborne illness aquired in the United States major pathogens. Emerg. Infect. Dis. 2011; 17: 7–15.

3. Jackson B.R., Griffin P.M., Cole D., Walsh K.A., Chai S.J.. Outbreak – associated Salmonella Еnterica Serotypes and Food Commodities United States, 1990–2008. Emer. Infect. Dis. 2013; 19(8): 1239–44.

4. Herikstad M., Motarjemi V., Tauxe R.V. Salmonella Surveilance: a global survey of public health serotyping. Epid. Infect. 2002; 129(1): 1–8.

5. [Information Bulletin of the Reference center for monitoring of salmonellosis]. 2016; (28): 1–70. (In Russ.).

6. Kulikovskiy G.A. [Salmonella: monitoring is needed]. Zhivotnovodstvo Rossii 2015; (6): 17– 8. (In Russ.).

7. Deng X., Ran L., Wu S., Ke B., He D., Yang X., Zhang Y., Ke C., Klena J.D., Yan M., Feng Z., Kan B., Liu X., Mikoleit M., Varma J.K. Laboratory-based surveillance of non-typhoidal Salmonella infections in Guangdong Province, China – 2009. In: International Conference on Emerging Infections Diseases. 11–14 July 2010, Atlanta, USA. Atlanta, 2010: 141.

8. The European Union Summery report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2014. EFSA Journal 2016; 14(2): 4380. DOI: 10.2903/j.efsa.2016.4380. (https://www.efsa.europa. eu/ en/efsajournal/pub/4380

9. Fakhr M.K., Nolan L.K., Loque C.V. Multilocus Sequence Typing Lacks the Discriminatory Ability of Pulsed-Field Gel Electrophoresis for Typing Salmonella enterica serovar Typhimurium. J. Clin. Microbiol. 2005; 43: 2215–19.

10. Haritonova N.E., Kuleshov K.V., Rozhnova S.Sh., Khristyukhina O.A., Podkolzin A.T. [The study of the genetic heterogeneity of isolates of Salmonella spp., Shigella spp. highlighted in the foci of the group and of sporadic disease in the Russian Federation. Proceedings of the VIII all-Russian scientific-practical conference with international participation «Molecular diagnostics – 2014».]. Moscow: MBA Publ., 2014; (1): 372–3. (In Russ.). http://www.epid-oki.ru/ images/documents/MD_2014_Kuleshov1.pdf

11. Kuleshov K.V., Fadina O.A., Rozhnova S. Sh., Podkolzin A.T., Shipulin G.A. Investigation of clonal structure of Salmonella Enteritidis isolated from outbreaks by Pulsed-Field Gel Electrophoresis in the Russian Federation during 2010– 2011. In: International Conference on Emerging Infections Diseases. 11–14 March, Atlanta, USA. Atlanta, 2012: 175.


About the Autors


For сorrespondence:
Sofiya Sh. Rozhnova, BD, Leading Researcher, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare; Superintendent, Reference center for monitoring of salmonellosis
Address: 3a Novogireevskaya St., Moscow 111123, Russia
E-mail: SALM@pcr.ru
Information about the authors:
Anna N. Guseva, Junior Researcher, Lab. for Molecular Diagnosis and Epidemiology of Intestinal Infections, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare», Moscow, Russia; e-mail: anna302bis@mail.ru
Olga A. Khristyukhina, Researcher, Lab. for Molecular Diagnosis and Epidemiology of Intestinal Infections, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare», Moscow, Russia; e-mail: SALM@pcr.ru
Olga N. Vitkova, Cand. Vet. Sci., Head, Department for Expertise of Normative Technical Documentation, Veterinary Center, Moscow, Russia; е-mail: olgavitkova@mail.ru
Natalia E. Krutova, Junior Researcher, Lab. for Molecular Diagnosis and Epidemiology of Intestinal Infections, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare, Moscow, Russia; e-mail: SALM@pcr.ru


Similar Articles


Бионика Медиа