Русский
Experience of using continuous spectrum pulsed UV radiation of plasma xenon lamps for decontamination of PCR products
DOI: https://dx.doi.org/10.18565/epidem.2025.15.1.51-55
Tutelyan A.V., Cherkashin E.A., Zamotaeva T.L., Abrosimova O.A., Potapenko A.O., Shashkovsky S.G., Goldshtein Ya.A., Akimkin V.G.
1) Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; 2) Entreprise de recherche et de production «Melitta», Moscow, Russia
Materials and methods. Using a pulsed UV source, experiments were conducted on 117 bp DNA amplicons. The efficiency of decontamination was assessed based on the residual amount of amplicons in washes from work surfaces such as plastic, glass, and metal.
Results. High efficiency of pulsed UV radiation in combating DNA contamination was demonstrated, and the optimal irradiation time (10–12 min), sufficient for complete destruction of amplicons on various types of surfaces (plastic, glass, metal) was determined. The data obtained make it possible to reduce the time of room treatment and reduce the negative impact of UV radiation on laboratory equipment and premises.
Conclusion. The feasibility of using pulsed units generating high-intensity continuous spectrum UV radiation flows for the destruction of amplicons on various surfaces was demonstrated.
Literature
1. Kleppe K., Ohtsuka E., Kleppe R., Molineux I., Khorana H.G. Studies on polynucleotides. XCVI. Repair replications of short synthetic DNA’s as catalyzed by DNA polymerases. J. Mol. Biol. 1971; 56: 341–361. DOI: 10.1016/0022-2836(71)90469-4
2. Saiki R., Scharf S., Faloona F., Mullis K., Horn G., Erlich H. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 1985; 230: 1350–1354. DOI: 10.1126/science.2999980
3. Краснов Я.М., Гусева Н.П., Шарапова Н.А., Черкасов А.В. Современные методы секвенирования ДНК. Проблемы особо опасных инфекций 2014; (2): 73–79. DOI: 10.21055/0370-1069-2014-2
Krasnov Ya.M., Guseva N.P., Sharapova N.A., Cherkasov A.V. [Modern methods of DNA sequencing]. Problems of Particularly Dangerous Infections 2014; (2): 73–79. (In Russ.). DOI: 10.21055/0370-1069-2014-2
4. Wong Y., Othman S., Lau Y., Radu S., Chee H. Loop-mediated isothermal amplifcation (LAMP): a versatile technique for detection of micro-organisms. J. Appl. Microbiol. 2018; 124(3): 626–643. DOI: 10.1111/jam.13647
5. Zhou L., Chandrasekaran A.R., Punnoose J.A., Bonenfant G., Charles S., Levchenko O. et al. Programmable low-cost DNA-based platform for viral RNA detection. Sci. Adv. 2020; 6(39): eabc6246. DOI: 10.1126/sciadv.abc6246
6. Волков А.Н., Начева Л.В., Захарова Ю.В. Молекулярно-генетические методы в практике современных медико-биологических исследований. Часть II: использование ПЦР в диагностике инфекционных заболеваний человека. Фундаментальная и клиническая медицина 2021; 6(1): 77–85. DOI: 10.23946/2500-0764-2021-6-1-77-85
Volkov A.N., Nacheva L.V., Zakharova Y.V. [Molecular genetic techniques in current biomedical research. Part II: PCR applications in diagnostics of human infectious diseases]. Fundamental and Clinical Medicine 2021; 6(1): 77–85. (In Russ.). DOI: 10.23946/2500-0764-2021-6-1-77-85
7. Кузин В.В., Колупаева Н.В., Щербакова О.А., Дятлов И.А. Деконтаминация поверхностей от нуклеиновых кислот аэрозолями дезинфицирующих средств. Microbiology Independent Research Journal 2023; 10(1): 1–12. DOI: 10.18527/2500-2236-2023-10-1-1-12
Kuzin V.V., Kolupaeva N.V., Shcherbakova O.A., Dyatlov I.A. [Decontamination of surfaces from nucleic acids with aerosols of disinfectants]. Microbiology Independent Research Journal 2023; 10(1): 1–12. (In Russ.). DOI:10.18527/2500-2236-2023-10-1-1-12
8. Jinno Y., Yoshiuraand K., Niikawa N. Use of psoralen as extinguisher of contaminated DNA in PCR. Nucleic Acids Res. 1990; 18(22): 6739 DOI: 10.1093/nar/18.22.6739
9. Алиев А.А., Шапиев Б.И., Алиев Н.А. Коррозионная активность дезинфицирующего средства для обработки помещений и объектов на основе нейтрального анолита. Известия Дагестанского государственного педагогического университета. Серия: Естественные и точные науки 2018; 12(4): 5–10. DOI:10.31161/1995-0675-2018-12-4-5-10
Aliev A.A., Shapiev B.I., Aliev N.A. [Corrosive activity of a disinfectant for treating premises and objects based on neutral anolyte]. News of the Dagestan State Pedagogical University. Series: Natural and Exact Sciences 2018; 12(4): 5–10. (In Russ.). DOI:10.31161/1995-0675-2018-12-4-5-10
10. Mwangi P., Mogotsi M., Ogunbayo A., Mooko T., Maringa W. A decontamination strategy for resolving SARS-CoV-2 amplicon contamination in a next-generation sequencing laboratory. Arch. Virol. 2022; 167(4): 1175–1179. DOI:10.1007/s00705-022-05411-z
11. Champlot S., Berthelot C., Pruvost M. An Efficient Multistrategy DNA Decontamination Procedure of PCR Reagents for Hypersensitive PCR Applications. PLoS One 2010; 5(9):e13042. DOI: 10.1371/journal.pone.0013042
12. Волынкина А.С., Рязанова А.Г., Русанова Д.В., Куличенко А.Н. Проблема ДНК (РНК)-контаминации в лаборатории при проведении диагностики COVID-19 методом ПЦР. Здоровье населения и среда обитания 2021; (7): 76–81. DOI: 10.35627/2219-5238/2021-29-7-76-81
Volynkina A.S., Ryazanova A.G., Rusanova D.V., Kulichenko A.N. [The problem of DNA (RNA) contamination in the laboratory when diagnosing COVID-19 using the PCR method]. Population Health and Environment 2021; (7): 76–81. (In Russ.). DOI: 10.35627/2219-5238/2021-29-7-76-81
13. Karlen Y., McNair A., Perseguers S., Mazza C., Mermod N. Statistical significance of quantitative PCR. BMC Bioinformatics 2007; 20(8): 131. DOI: 10.1186/1471-2105-8-131
14. Sicairos-Ruelas E.E., Gerba C.P., Bright K.R. Efficacy of copper and silver as residual disinfectants in drinking water. J. Environ. Sci. Health A Tox. Hazard Subst. Environ. Eng. 2019; 54(2): 146–155. DOI: 10.1080/10934529.2018.1535160
15. Farrer A.G., Wright S., Skelly E., Eisenhofer R., Dobney K., Weyrich L. Effectiveness of decontamination protocols when analyzing ancient DNA preserved in dental calculus. Sci. Rep. 2021; 11(1): 7456. DOI: 10.1038/s41598-021-86100-w
16. Soro A., Ekhlas D., Shokri S., Yem M., Li R., Barroug S. The efficiency of UV light-emitting diodes (UV-LED) in decontaminating Campylobacter and Salmonella and natural microbiota in chicken breast, compared to a UV pilot-plant scale device. Food Microbiology 2023; 116: 104365. DOI: 10.1016/j.fm.2023.104365
About the Autors
Professor Aleksey V. Tutelyan, Corresponding Member of the Russian Academy of Sciences, МD, Head, Laboratory of Infections Related to Medical Cares, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; bio-tav@yandex.ru; http://orcid.org/0000-0002-2706-6689
Evgeny A. Cherkashin, Cand. Chem. Sci., Head, Center for Product Development, Innovation and Development, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; e.cherkashin@pcr.ms; http://orcid.org/0000-0002-3627-6047
Tatiana L. Zamotaeva, Researcher, Center for Product Development, Innovation and Development, Center for Development, Product Development and Innovation, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; zamotaevatat@gmail.com; http://orcid.org/0009-0003-9799-3749
Olga A. Abrosimova, Epidemiologist, Head, Epidemiological Service, Center for Development, Product Development and Innovation, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; kvasova@cmd.su; http://orcid.org/0000-0002-4545-1804
Aleksey O. Potapenko, Researcher, Entreprise de recherche et de production «Melitta», Moscow, Russia; alexeu1999pt@gmail.com
Sergey G. Shashkovsky, Cand. Techn. Sci., Chief Designer, Entreprise de recherche et de production «Melitta», Moscow, Russia; melitta916@gmail.com
Yakov A. Goldshtein, General Director, Entreprise de recherche et de production «Melitta»,, Moscow, Russia; yagmed@gmail.com
Professor Vasily G. Akimkin, Academician of the Russian Academy of Sciences, MD, Director, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; vgakimkin@yandex.ru; https://orcid. org/0000-0003-4228-9044
Similar Articles
