Clinical strains of Vibrio cholerae neO1/neO139 serogroups in Russia: Isolation dynamics, serological belonging, genetic features


DOI: https://dx.doi.org/10.18565/epidem.2021.11.1.52-6

Arkhangelskaya I.V., Kruglikov V.D., Levchenko D.A., Monakhova E.V., Nepomnyashchaya N.B., Podoinitsyna O.A., Ezhova M.I.

Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia
Objective. To investigate the detection dynamics and the serological and molecular genetic features of clinical strains of V. cholera popO1/popO139 serogroups isolated in the subjects of the Russian Federation in 2014–2018.
Materials and methods. A total of 40 clinical V. cholera nonO1/nonO139 cultures isolated from patients in 6 subjects of the Russian Federation were studied. Bacteriological, serological, and genetic studies were used.
Results. The belonging of Vibrio cholerae to 14 serogroups was established; the circulations of specific serogroups in different territories were determined. The strains had different sets of genetic determinants of pathogenicity/persistence factors.
Conclusion. The strains of vibrio cholerae neO1/neO139 serogroups, both isolated from patients in the southern regions of Russia and imported to other subjects from the countries of Southeast Asia and Africa, were characterized by variability in biological properties. Their genetic features are associated, among other things, with the presence of the heat-stable toxin gene (stn) in 4 strains.

Literature


1. Blokesch M., Schoolnik G.K. Serogroup conversion of Vibrio cholerae in aquatic reservoirs. PLoS Pathog. 2007; 3(6): e81. doi:10.1371/journal. ppat.0030081


2. Grigorenko L.V., Kruglikov V.D., Mazrukho A.B., Arkhangel’skaya I.V., Zubkova D.A., Nepomnyashchaya N.B., Shestialtynova I.S. [Cholera vibrios non-O1/non-O139 isolated in the process of epidemiological monitoring over Rostov-on-Don water basins and drain sewage system within the period of 2009–2011]. Problemy osobo opasnykh infektsiy 2013; (4): 48–50. (In Russ.). https://doi.org/10.21055/0370-1069-2013-4-48-50.


3. Li F., Du P., Li B., Ke C., Chen A., Chen J., Zhou H., Li J., Morris J.G., Kan B., Wang D. Distribution of virulenceassociated genes and genetic relationships in non-O1/O139 Vibrio choleraе aquatic isolates from China. Appl. Environ. Microbiol. 2014; 80(16): 4987–92. doi: 10.1128/AEM.01021-14


4. Marin M.A., Thompson C.C., Freitas F.S., Finseca E.L., Aboderin A.O., Zailani S.B., Quartey N.K., Okeke I.N., Vicente A.C. Cholera outbreaks in Nigeria are associated with multidrug resistant atypical El Tor and non-O1/non-O139 Vibrio choleraе. PLoS Neg l. Trop. Dis. 2013; 7(2): 2049. doi: 10.1371/journal.pntd.0002049


5. Schirmeister F., Diekmann R., Bechlars S. et al. Genetic and phenotypic analysis of Vibrio cholerae non-O1, non-O139 isolated from German and Austrian patients. Eur. J. Clin. Мicrobiol. Infect. Dis. 2014; (33): 767–78. doi: 10.1007/s10096-013-2011-9


6. Zmeter C., Tabaya H., Sharara A.I., Kanj S.S. Non-O1, non-O139 Vibrio cholerae septicemia at a tertiary care center in Beirut, Lebanon; a case report and review. J. Infect. Public. Health. 2018; 11(5): 601–4. doi: 10.1016/j.jiph.2018.01.001


7. Shanley J., Kanj A., El Zein S., Tabaja H., Trzcinski B., Horman J., Salimnia H., Fairfax M., Singh M. Non-O1, non-O139 Vibrio cholerae bacteremia in an urban academic medical center in the United States. IDCases 2019; (5); e00527. https://doi.org/10.1016/j.idcr.2019.e00527


8. Monakhova E.V., Arkhangel’skaya I.V. [Cholera vibrios of nonO1/nonO139 serogroups in etiology of acute intestinal infections: current situation in Russia and around the world]. Problemy osobo opasnykh infektsiy 2016; (2): 14–23. (In Russ.). https://doi.org/10.21055/0370-1069-2016-2-14-23


9. Chatterjee S., Ghosh K., Raychoudhuri A., Chowdhury G., Bhattacharya M.K., Mukhopadhyay A.K., Ramamurthy T., Bhattacharya S.K., Klose K.E., Nandy R.K. Incidence, virulence factors, and clonality among clinical strains of non-O1, non-O139 Vibrio cholerae isolates from hospitalized diarrheal patients in Kolkata, India. J. Clin. Microbiol. 2009; 47(4): 1087–95. doi:10.1128/JCM.02026-08


10. Hasan N.A., Choi S.Y., Eppinger M., Clark P.W., Chen A., Alam M., Haley B.J., Taviani E., Hine E., Su Q., Tallon L.J., Prosper J.B., Furth K., Hoq M.M., Li H., Fraser-Liggett C.M., Cravioto A., Huq A., Ravel J., Cebula T.A., Colwell R.R. Genomic diversity of 2010 Haitian cholera outbreak strains. Proc. Natl. Acad. Sci. USA 2012; 109(29): 2010–17. doi:10.1073/pnas.120735910.


11. Selyanskaya N.A., Arkhangel’skaya I.V., Vodop’yanov A.S., Vodop’yanov S.O., Kruglikov V.D., Vodyanickaya S.Yu., Verkina L.M., Nepomnyashchaya N.B. [Typing of Vibrio сholerae nonO1/nonO139 strains isolated in Rostov region in 2014]. Zhurnal mikrobiologii, epidemiologii i immunobiologii 2016; (1): 3–9. (In Russ.). https://doi.org/10.36233/0372-9311-2016-1-3-9


12. Telesmanitch N.R., Chaika S.O., Chaika I.A., Goncharenko E.V., Lomov Yu.M. [The mass-spectrometric analysis of MALDITOF in identification and typing of strains of comma bacillus]. Klinicheskaya i laboratornaya diagnostika 2016; 61(6): 375–379. (In Russ.). doi:10.18821/0869-2084-2016-61-6-375-379


13. Monakhova E.V., Arkhangel’skaya I.V., Pisanov R.V., Titova S.V. [Peculiarities of primary MARTX cytotoxin structure in nontoxigenic Vibrio cholerae strains of different serogroups]. Problemy osobo opasnykh infektsiy 2018; (3): 73–7. (In Russ.). https://doi.org/10.21055/0370-1069-2018-3-73-77


14. Monakhova E.V., Arkhangel’skaya I.V., Titova S.V., Pisanov R.V. [MSHA-like pili of non-toxigenic Vibrio cholerae strains Rostov-on-Don]. Problemy osobo opasnykh infektsiy 2019; (3): 75–80. (In Russ.). https://doi.org/10.21055/0370-1069-2019-3-75-80


15. Caldini G., Neri A., Cresti S., Boddi V., Rossolini G.M., Lanciotti E. High prevalence of Vibrio cholerae non-O1 carrying heat-stable-enterotoxin-encoding genes among Vibrio isolates from a temperate-climate river basin of central Italy. Appl. Environ. Microbiol. 1997; 63(7): 2934–9.


16. Octavia S., Salim A., Kurniawan J., Lam C., Leung Q., Ahsan S., Reeves P.R., N air G.B., Lan R. Population structure and evolution of non-o1/non-O139 Vibrio cholerae by multilocus sequence typing. PLoS One 2013; 8(6): 65342. doi:10.1371/journal.pone.0065342


17. Ceccarelli D., Chen A., Hasan N.A., Rashed S.M., Huq A., Colwell R.R.. Non-O1/non-O139 Vibrio cholerae carrying multiple virulence factors and V.cholerae O1 in the Chesapeake Bay, Maryland. Appl. Environ Microbiol. 2015; 81: 1909–18. doi:10.1128/AEM.03540-14.


About the Autors


Irina V. Arkhangelskaya, Cand. Med. Sci., Researcher, Laboratory of Cholera Microbiology, Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; irina070769@mail.ru; http://orcid.org/0000-0002-7569-8584
Vladimir D. Kruglikov, MD, Head, Laboratory of Cholera Microbiology, Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; kruglikov_vd@antiplague.ru; http://orcid.org/0000-0003-4749-3837
Daria A. Levchenko, Cand. Med. Sci., Senior Researcher, Laboratory of Cholera Microbiology, Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; da@antiplague.ru; http://orcid.org//0000-0002-5073-2918
Elena V. Monakhova, Biol.D. Head, Group of Molecular Biology, Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; unicorn54@mail.ru; http://orcid.org/0000-0002-9216-7777
Natalia B. Nepomnyashchaya, Researcher, Group of Molecular Biology, Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; nepomnyaschaya_nb@antiplague.ru; http://orcid.org/0000-0003-0868-6791
Oksana A. Podoinitsyna, Researcher, Laboratory of Cholera Microbiology, Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; oksankashalu@yandex.ru; https://orcid.org/0000-0002-9996-4189
Maria I. Ezhova, Researcher, Laboratory of Cholera Microbiology, Rostov-on-Don Plague Control Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; mari-ezho@yandex.ru; http://orcid.org/0000-0003-4254-3313


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