Русский
Comparison of the results of molecular biological and bacteriological studies for the identification of methicillin-resistant Staphylococcus strains in bacteremia
DOI: https://dx.doi.org/10.18565/epidem.2021.11.1.48-51
Skachkova T.S., Lashenkova N.N., Fomina V.S., Zamyatin M.N., Gusarov V.G., Dementienko M.V., Shipulina O. Yu., Goloveshkina E.N., Akimkin V.G.
1) Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; 2) N.I. Pirogov National Medical and Surgical Center, Ministry of Health of Russia, Moscow, Russia
Subjects and methods. A parallel bacteriological and molecular biological (real-time PCR) study was conducted to examine biomaterials from 240 patients with signs of infection who were treated in a Moscow multidisciplinary hospital. The biological material was peripheral vein blood taken in vacuum test tubes with EDTA for PCR and in vials containing a nutrient medium for bacteriological testing.
Results. The sensitivity and specificity of the PCR assay versus the bacteriological test in detecting blood methicillin-resistant staphylococci were 100 and 97%, respectively. Methicillin-resistant staphylococci were detected by PCR in 6.3% of patients with signs of infection and by bacteriological tests in 3.3% of patients.
Conclusion. The real-time PCR assay used for the detection of blood methicillin-resistant staphylococci will be able to reduce the time of a diagnostic study, to increase the possibility of identifying the etiological agent for bacteremia, and to timely perform adequate antimicrobial therapy.
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About the Autors
Tatiana S. Skachkova, Researcher, Department of Molecular Diagnosis and Epidemiology, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; skachkova@inbox.ru; https://orcid.org/0000-0003-1924-6521
Natalia N. Lashenkova, Bacteriologist, Laboratory of Bacteriology, N.I. Pirogov National Medical and Surgical Center, Moscow, Russia; lashenkovann@ pirogov-center.ru
Valeria S. Fomina, Cand. Med. Sci., Chief, Service of Clinical Laboratory Diagnosis, N.I. Pirogov National Medical and Surgical Center, Moscow, Russia; FominaVS@pirogov-center.ru
Professor Mikhail N. Zamyatin, MD; Head, Department of Anesthesiology and Resuscitation, N.I. Pirogov National Medical and Surgical Center, Moscow, Russia; ZamyatinMN@pirogov-center.ru; https://orcid.org/0000-0002-2072-7798
Vitaly G. Gusarov, Cand. Med. Sci., Head, Department of Anesthesiology and Resuscitation (Intensive Care), N.I. Pirogov National Medical and Surgical Center, Moscow, Russia; gusarov1974@mail.ru; https://orcid.org/0000-0002-2900-1459
Maria V. Dementienko, Anesthesiologist/Resuscitation Specialist, N.I. Pirogov National Medical and Surgical Center, Moscow, Russia; dementienkomv@pirogov-center.ru
Olga Yu. Shipulina, Cand. Med. Sci., Head, Laboratory of Molecular Studies, Department of Molecular Diagnosis and Epidemiology, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; olga.shipulina@pcr.ms; https://orcid.org/0000-0003-4679-6772
Elena N. Goloveshkina, Cand. Biol. Sci., Head, Laboratory of Molecular Diagnosis and Epidemiology of Reproductive Organ Infections, Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; goloveshkina@cmd.su; https://orcid.org/0000-0002-0536-2874
Professor Vasily G. Akimkin, MD, Academicien of the Russian Academy of Sciences, Director, Central Research Institute for Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Moscow, Russia; vgakimkin@yandex.ru;: https://orcid.org/0000-0003-4228-9044.
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