Monitoring the cytogenetic consequences of tick-borne encephalitis occurring with opisthorchiasis invasion due to glutathione-S-transferase gene polymorphisms GSTM1 and GSTTA1


DOI: https://dx.doi.org/10.18565/epidem.2020.10.1.75-9

Ilyinskikh N.N., Ilyinskikh E.N., Kostromeeva M.V., Filatova E.N.

1) National Research Tomsk State University, Ministry of Education and Science, Tomsk, Russia; 2) Siberian State Medical University, Ministry of Health of Russia, Tomsk, Russia
Objective. To investigate the duration of the preserved cytogenetic consequences of acute tick-borne encephalitis (TBE) in patients having background chronic infection with Opisthorchis felineus according to the polymorphism of glutathione-S-transferase enzyme genes.
Subjects and methods. The investigation enrolled 103 patients with TBE having background chronic opisthorchiasis (CO), 74 TBE patients without CO, 78 patients with CO, and 75 healthy donors, in whom a T lymphocyte cytokinesis-blocked micronucleus assay was performed and the GSTM1 and GSTT1 gene variants were determined using PCR.
Results. The levels of lymphocytes with micronuclei (MN) in TBE patients with CO were significantly lower than in those without CO. However, in these patients, unlike the other groups, the number of cells with MN did not decrease to the control level within 6 months (a follow-up period). In this group, no relationship could be either established between the level of lymphocytes with MN and the polymorphism of the glutathione-S-transferase enzyme genes.
Conclusion. The elevated levels of cytogenetic abnormalities in TBE patients with CO persist longer than in those without CO, which allows them to be identified as a group at risk of developing immunodeficiency and complications.
Keywords: glutathione-S-transferase, micronuclear assay, tick-borne encephalitis, opisthorchiasis, GSTT1, GSTM1, gene polymorphism
Read entire article

Literature


  1. Chard A.N., Baker K.K., Tsai K., Levy K., Sistrunk J.R., Chang H.H., Freeman MC. Associations between soil-transmitted helminthiasis and viral, bacterial, and protozoal enteroinfections: a cross-sectional study in rural Laos. Parasit. Vectors 2019; 12(1): 216. DOI: 10.1186/s13071-019-3471-2

  2. Donohue R.E., Cross Z.K., Michael E. The extent, nature, and pathogenic consequences of helminth polyparasitism in humans: A meta-analysis. PloS Negl. Trop. Dis. 2019; 13(6): e0007455. DOI: 10.1371/journal.pntd.0007455.

  3. Griffiths E.C., Pedersen A.B., Fenton A., Petchey O.L. Analysis of a summary network of co-infection in humans reveals that parasites interact most via shared resources. Proc. Biol. Sci. 2014; 281(1782): 20132286. DOI: 10.1098/rspb.2013.2286

  4. Fedorova O.S., Fedotova M.M., Sokolova T.S., Golovach E.A., Kovshirina Y.V., Ageeva T.S., Kovshirina A.E., Kobyakova O.S., Ogorodova L.M., Odermatt P. Opisthorchis felineus infection prevalence in Western Siberia: A review of Russian literature. Acta Trop. 2018; 178: 196−204. DOI: 10.1016/j.actatropica.2017.11.018

  5. Ильинских Н.Н., Ильинских Е.Н., Костромеева М.В. Полиморфизм кариопатологических последствий инвазии Opisthorchis felineus (Rivolta, 1884) у различных этнических групп коренных и пришлых жителей на севере Западной Сибири. Медицинская паразитология и паразитарные болезни 2018; (1): 13−8.

    Ilyinskikh N.N., Ilyinskikh E.N., Kostromeeva M.V. [Polymorphism of the karyopathological consequences of Opisthorchis felineus (Rivolta, 1884) invasion in different ethnic groups of indigenous and immigrant inhabitants in the north of Western Siberia]. Medicinskaуa parazitologiуa I parazitarnye bolezni 2018; (1): 13−8. (In Russ.).



  6. Promthet S., Songserm N., Woradet S., Pientong C., Ekalaksananan T., Wiangnon S., Ali A. Opisthorchiasis with proinflammatory cytokines (IL-1β and TNF-α) polymorphisms influence risk of intrahepatic cholangiocarcinoma in Thailand: a nested case-control study. BMC Cancer 2018; 18(1): 846. DOI: 10.1186/s12885-018-4751-5

  7. Kaewraemruaen C., Sermswan R.W., Wongratanacheewin S. Induction of regulatory T cells by Opisthorchis viverrini. Parasite Immunol. 2016; 38(11): 688−97. DOI: 10.1111/pim.12358

  8. Ананьева О.В., Ильинских Н.Н., Ильинских Е.Н., Замятина Е.В., Дуров А.М. Изучение частоты микроядер в эпителиоцитах и лимфоцитах в зависимости от полиморфизма генов глутатион-S-трансферазы у больных клещевым энцефалитом. Морфология 2018; 154(6): 7−11.

    Anan’eva O.V., Ilyinskikh N.N., Ilyinskikh E.N., Zamyatina E.V., Durov A.M. [Analysis of micronuclei frequency in epithelial cells and lymphocytes and its correlation with glutathione-S-transferase gene polymorphism in patients with tick-borne encephalitis]. Morfologiya 2018; 154(6): 7−11. (In Russ.).



  9. Bolognesi C., Fenech M. Micronucleus assay in human cells: lymphocytes and buccal cells. Methods Mol. Biol. 2013; 1044: 191–207. DOI: 10.1007/978-1-62703-529-3_10.

  10. Bigatti M. P., Santovito A. Glutathione S-transferase T1 (GSTT1) and M1 (GSTM1) polymorphisms in a sample of the population in Northern Italy. Genetika 2007; 43(6): 827–830.

  11. Ильинских Н.Н., Ксенц А.С., Ильинских Е.Н., Манских В.Н., Васильев С.А., Ильинских И.Н. Микроядерный анализ в оценке цитогенетической нестабильности. Томск: Издательство Томского государственного педагогического университета, 2011. 234 с.

    Ilyinskikh N.N., Ksents A.S., Ilyinskikh E.N., Manskikh V.N., Vasilyev S.A., Ilyinskikh I.N. [Micronucleous test in cytogenetic instability assessment]. Tomsk: Izdatel’stvo Tomskogo Gosudarstvennogo Pedagogicheskogo Universiteta, 2011.234 p. (In Russ.).



  12. Leite S.T., Silva M.B., Pepato M.A., Souto F.J., Santos R.A., Bassi-Branco C.L. Increased frequency of micronuclei in the lymphocytes of patients chronically infected with hepatitis B or hepatitis C virus. Mem. Inst. Oswaldo Cruz. 2014; 109(1): 15−20. DOI: 10.1590/0074-0276140183

  13. Bhattacharya S.K., Saraswathy R., Sivakumar E. Genotoxic assessment in peripheral blood lymphocytes of post-polio individuals using sister chromatid exchange analysis and micronucleus assay. Hum. Exp. Toxicol. 2011; 30(7): 636–48. DOI: 10.1177/0960327110376983


About the Autors

Prof. Nikolay N. Ilyinskikh, BD, Professor, Department of Biology and Genetics, Siberian State Medical University, Ministry of Health of Russia; Professor, Department of Ecology, Nature Management, and Environmental Engineering; National Research Tomsk State University, Ministry of Education and Science of Russia, Tomsk, Russia; e-mail: nauka-tomsk@yandex.ru; ORCID: http://orcid.org/0000-0003-1014-1096
Ekaterina N. Ilyinskikh, MD, Assistant Professor, Professor, Department of Infectious Diseases and Epidemiology, Siberian State Medical University, Ministry of Health of Russia; Professor, Department of Ecology, Nature Management, and Environmental Engineering, National Research Tomsk State University, Ministry of Education and Science of Russia, Tomsk, Russia; e-mail: infconf2009@mail.ru; ОRCID: http://orcid.org/0000-0001-7646-6905
Marina V. Kostromeeva, Postgraduate Student, Department of Ecology, Nature Management, and Environmental Engineering, National Research Tomsk State University, Ministry of Education and Science of Russia, Tomsk, Russia; e-mail: kostromeeva2018@mail.ru; ORCID: http://orcid.org/0000-0002-8212-7181
Evgenia N. Filatova, Postgraduate Student, Department of Infectious Diseases and Epidemiology, Siberian State Medical University, Ministry of Health of Russia, Tomsk, Russia; e-mail: synamber@mail.ru


Similar Articles

Back to Content

Бионика Медиа