PCR genotyping of non-toxigenic Vibrio cholerae strains as one of approaches to their actualization in terms of epidemiological surveillance of cholera


DOI: https://dx.doi.org/10.18565/epidem.2018.2.28-35

Kruglikov V.D., Levchenko D.A., Vodopyanov A.S., Nepomnyashchaya N.B.

Rostov-on-Don Anti-Рlague Research Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia;
Objective. To carry out PCR genotyping (with a high discriminating power) of non-toxigenic Vibrio cholerae strains isolated from environmental objects.
Materials and methods. The authors carried out PCR genotyping of a representative sample from 408 non-toxigenic V. cholerae strains isolated from water bodies in the Russian Federation in 1989 to 2016, which was based on the detection of the calculated optimal number (n = 14) of virulence factor genes. A cluster analysis by the unweighted pair group method with arithmetic mean (UPGMA) was used to distribute V. cholerae isolates in 81 genotypes on the basis of different combinations (presence/absence) of these genes.
Results. Genotype G10 strains were ascertained to be isolated from the Agur River in the Krasnodar Territory in different years (1993, 1999, 2007), which was suggestive of their persistent potential. In 2015, the likely drift was 89 strains of the other cluster (genotype D4). In addition, 65 V. cholerae ctxA-tcpA+ strains belonging to 26 genotypes were included in both separate clusters and in common ones with ctxA-tcpA-cultures.
Conclusion. The proposed approach assists in systematizing the non-toxigenic strains of V. cholerae in terms of establishing (with a high discriminating power) their possible origin, as well as their likely importance in the etiology of acute intestinal infections, which is an important component of epidemiological investigations.

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For citations: Kruglikov V.D., Levchenko D.A., Vodopyanov A.S., Nepomnyashchaya N.B. PCR genotyping of nontoxigenic Vibrio cholerae strains as one of approaches to their actualization in terms of epidemiological surveillance of cholera. Èpidemiologiâ i infekcionnye bolezni. Аktual’nye voprosy 2018; (2):28–35


About the Autors


For correspondence:
Darya A. Levchenko, Junior Researcher, Laboratory of Cholera Microbiology, Rostov-on-Don Research Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Address: 117/40, Maxim Gorky Street, Rostov-on-Don 344002, Russia
Теlеphоne: +7 (863) 240-91-33
Е-mail: levchenko_da@antiplague.ru
Information about the authors:
Vladimir D. Kruglikov, MD, Head, Laboratory of Cholera Microbiology, Rostov-on-Don Research Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; e-mail: kruglikov_vd@antiplague.ru
Aleksey S. Vodopyanov, Cand. Med. Sci., Senior Researcher, Head, Group of Virology, Rostov-on-Don Research Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; e-mail: vodopyanov_as@antiplague.ru
Natalia B. Nepomnyaschaya, Researcher,  Group of Molecular Biology, Rostov-on-Don Research Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Rostov-on-Don, Russia; e-mail: nepomnyaschaya _nb@antiplague.ru


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