Русский
Experimental study of the effect of the active substance of miramistin® on the infectious activity of SARS-CoV-2 in vitro
DOI: https://dx.doi.org/10.18565/epidem.2022.12.3.91-6
Leneva I.A., Gracheva A.V., Korchevaya E.R., Smirnova D.I., Faizuloev E.V.
I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
Materials and methods. The investigators used cultured Vero CCL-81 cells derived from the monkey kidney and the laboratory SARS-CoV-2 strain Dubrovka. They applied cultural, virological, molecular biological, and statistical studies. All experiments used the original pharmaceutical substance BMPA chloride (OOO «INFAMED», Russia).
Results. The study of BMPA cytotoxicity in the cultured cells indicated that its CTD50 was 0,0091±0,002%, and the MTC was 0.005%. The original drug had a pronounced virucidal activity against SARS-CoV-2, by completely suppressing its infectivity at a concentration of 0.005% or higher. An additional experiment identifying the result by virus titration and in the virus-specific real-time RT-PCR assay confirmed complete virus inactivation in the range of non-toxic concentrations of 0.01–0.0025%.
Conclusion. The original pharmaceutical substance BMPA chloride in vitro exhibits a pronounced virucidal activity at the concentrations corresponding to that of the active substance in miramistin (0.01%), and even at lower concentrations (0.005 and 0.0025%).
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About the Autors
Irina A. Leneva, BiolD, Head, Laboratory of Experimental Virology, I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia; wnyfd385@yandex.ru; https://orcid.org/0000-0002-7755-2714
Anastasia V. Gracheva, Junior Researcher, Laboratory of Molecular Virology, I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia; anastasiia.gracheva. 95@mail.ru; https://orcid.org/0000-0001-8428-4482
Ekaterina R. Korchevaya, Junior Researcher, Laboratory of Molecular Virology, I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia; c.korchevaya@gmail.com; https://orcid.org/0000-0002-6417-3301
Daria I. Smirnova, Junior Researcher, Laboratory of Molecular Virology, I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia; daria.sm.1995@mail.ru; https://orcid.org/0000-0001-7325-0834
Evgeny B. Faizuloev, Cand. Biol. Sci., Head, Laboratory of Molecular Virology, I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia; faizuloev@mail.ru; https://orcid.org/0000-0001-7385-5083
Вклад авторов. Ленева И.А. – дизайн и проведение исследования, статистическая обработка данных, написание статьи; Грачева А.В. – дизайн и проведение исследования, написание статьи; Корчевая Е.Р. – проведение исследования, статистическая обработка результатов; Смирнова Д.И. – проведение исследования; Файзулоев Е.Б. – дизайн и проведение исследования, редактирование статьи.
Authors contributions. Leneva I.A. – design and conduction of the investigation; statistical processing of the results; writing the article; Gracheva A.V. – design and conduction of the investigation; Korchevaya E.R. – conduction of the investigation; statistical processing of the results; Smirnova D.I. – conduction of the investigation; Fayzuloev E.B. – design and conduction of the investigation; editing the text.
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