New opportunities to diagnose HHV-8 infection by enzyme immunoassay


DOI: https://dx.doi.org/10.18565/epidem.2019.9.4.51-7

Amеlina Е.A., Arsеnyеva V.A., Mardanly S.S., Mardanly S.G., Rotanov S.V.

1) ECOlab, Elektrogorsk, Moscow Region, Russia; 2) Honorary Academician N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Moscow, Russia; 3) State University for Humanities and Technology, Orekhovo-Zuyevo, Moscow Region, Russia; 4) Moscow Research and Practical Center of Dermatovenereology and Cosmetology, Moscow Healthcare Department
Objective. To create a reagent kit for detecting specific IgG antibodies to human herpesvirus type 8 (HHV-8) by solid-phase enzyme-linked immunosorbent assay (ELISA).
Subjects and methods. ELISA was used to examine serum samples from HIV-infected, healthy donors, children, and elderly people for specific anti-HHV-8 antibodies, by confirming the results by immunofluorescence assay (IFA). The activity and cross-reactivity of a new reagent kit for detection of other herpesviruses were investigated.
Results. The created reagent set for the detection of specific IgG antibodies to HHV8 by ELISA corresponds to the declared values.
Conclusion. An ELISA-based diagnostic test will be able to rapidly screen a large number of sera for the identification of people at risk for Kaposi’s sarcoma and to conduct seroepidemiological studies.
Keywords: Kaposi’s sarcoma, herpes virus type 8, enzyme-linked immunosorbent assay
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About the Autors

Elena A. Amelina, Cand. Biol. Sci., Head, Department of Advanced Developments, ECOlab, Elektrogorsk, Moscow Region, Russia; e-mail: ekolab-ferment@mail.ru; ORCID: http:// orcid.org/0000-0001-6979-6631
Veronika A. Arsenyeva, Senior Microbiologist, Department of Advanced Developments, ECOlab, Elektrogorsk, Moscow Region, Russia; e-mail: ecolab.arsenyeva@gmail. com; ORCID: http://orcid.org/0000-0002-0517-2181
Sarkhan S. Mardanly, Junior Researcher, Department of Epidemiology, Honorary Academician N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russia; Deputy General Director for Commercial Affairs, EKOlab, Electrogorsk, Moscow Region, Russia; e-mail: ekolab-sarkhan@mail.ru; ORCID: http://orcid.org/0000-0002-4440-6075
Seyfaddin G. Mardanly, MD, Academician of the Russian Academy of Medical and Technical Sciences, Honored Health Care Worker of the Russian Federation; Professor, Department of Pharmacology and Pharmaceutical Sciences, Moscow State University for Humanities and Technology; President and Director of Science, ECOlab, Elektrogorsk, Moscow Region, Russia; e-mail: ekolab-president@mail.ru; ORCID: http://orcid.org/0000-0002-4556-135X
Sergey V. Rotanov, MD, Associate Professor, Leading Researcher, Moscow Research and Practical Center of Dermatovenereology and Cosmetology, Moscow Healthcare Department, Moscow, Russia; e-mail: svrotanov@mail.ru; ORCID: http://orcid.org/ 0000-0002-3222-1401

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